FastQCFastQC Report
Wed 25 May 2016
SRR1033222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866929
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114320.39875420702779873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.034.4728161
GTATCAA78300.029.3118761
ATCAACG96500.024.3405823
TCAACGC97250.024.0770724
CAACGCA99300.023.6492635
AACGCAG102100.023.001516
TATCAAC99950.022.7515092
ACGCAGA115550.020.0867847
CGCAGAG118400.019.3999848
AGAGTAC110500.019.17048811
GAGTACT74600.018.66942212
GTACATG79850.018.3780331
AGTACTT76300.018.16363713
CAGAGTA120350.017.83007610
GCAGAGT123850.017.5757889
GTACTTT79900.017.43836214
TACATGG80750.017.3531282
ATACCGC2802.6739144E-1017.19226
GTGGTAT23800.017.0950491
GTCGTAC2109.1936636E-716.5593071
Produced by FastQC (version 0.11.5)