FastQCFastQC Report
Wed 25 May 2016
SRR1033222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866929
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.036.5901031
GTATCAA78950.026.3880271
GTGGTAT24050.020.8302611
TCAACGC98200.020.680744
ATCAACG98250.020.6702163
TATAACG1551.0112617E-620.0204162
CAACGCA101450.020.018225
TATCAAC102600.019.9954812
AACGCAG105900.019.1444826
TGGTATC25150.018.5079382
GTACATG82650.017.30611
CGGCTAT2251.11675035E-716.85669530
TAAGCGC2251.11675035E-716.85669523
ACGCAGA121400.016.6433687
TACATGG82850.016.5635282
TGTCGCG1250.001949255816.5502097
ATACCGC3351.0913936E-1116.4678696
CGCAGAG122750.016.3760578
AGAGTAC117150.015.89356411
TCTACAC9900.015.6725463
Produced by FastQC (version 0.11.5)