FastQCFastQC Report
Wed 25 May 2016
SRR1033219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654603
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143190.3918072633333908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA3750.026.70315429
GTATCAA88550.023.9936081
GGTATCA59700.023.7062571
GTACATG105550.022.6988531
TACATGG106000.021.3213212
ACATGGG109500.020.7000893
ATCAACG104900.020.2306863
TCAACGC105550.020.1684994
CAACGCA107950.019.751965
ACGGAAC4950.019.5299366
AACGCAG111250.019.2905086
TATCAAC109150.019.1132342
CGCGTAA900.005837766519.10884510
GCGAACT5250.019.07447228
AGCGAAC5450.019.01413727
GAGTACA75150.017.7682691
CCAACGG5850.017.70350563
AGAGTAC112200.017.59648711
CATGGGG66400.016.7292164
ACGCAGA127800.016.6856777
Produced by FastQC (version 0.11.5)