FastQCFastQC Report
Wed 25 May 2016
SRR1033219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654603
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.025.3559651
GTATCAA97200.023.7794461
GTACATG105200.021.5770231
TACATGG104100.021.0656072
ATCAACG113850.020.0187053
TCAACGC113950.019.940624
CAACGCA117050.019.471425
ACATGGG107050.019.3577733
TATCAAC121450.018.9079422
AACGCAG121200.018.8900476
CGAACTA3051.8189894E-1218.08788129
GAGTACA76050.017.0362471
AGAGTAC122400.016.76135311
GTATAGG8450.016.3547951
TATACTG16050.016.326995
ACGCAGA141050.016.109427
CATGGGG65300.016.0519564
GAGTACT82300.016.0462112
CGCAGAG142600.015.6925218
AGTACTT83850.015.626224513
Produced by FastQC (version 0.11.5)