FastQCFastQC Report
Wed 25 May 2016
SRR1033218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437452
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94780.38884868296893643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.039.035741
GTATCAA67900.028.7834341
ATCAACG86250.023.253753
TCAACGC87100.023.1000964
CAACGCA89600.022.4171775
TATCAAC87250.022.2799072
AACGCAG92000.021.7963476
ACGCGCG800.00289329621.57154733
GTGGTAT19350.021.5663341
TGGTATC19650.019.9978312
CGGTTAT900.00569909819.1882124
ACGCAGA103900.019.1687097
CGCAGAG104950.019.0097058
GTACATG59600.018.6715241
AGAGTAC102700.017.88511311
TACATGG60550.017.4592842
CAGAGTA109050.017.22216810
GAGTACT76100.017.22111112
TCTGTCG4800.017.1988668
GCAGAGT111550.017.0212089
Produced by FastQC (version 0.11.5)