FastQCFastQC Report
Wed 25 May 2016
SRR1033218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437452
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.038.9249761
GTATCAA67700.026.1789441
CGAACTA1551.537046E-924.4706929
GTGGTAT18750.022.847771
TCAACGC82550.021.3028814
ATCAACG83450.021.114453
TGGTATC20300.020.7228282
CAACGCA85000.020.6888545
AACGCAG87450.020.2275266
TATCAAC88250.020.0832312
TACCGTC1409.394009E-619.7036727
AGCGAAC1952.1706E-819.4510627
GTACATG61700.017.9179481
GTATACG1351.561137E-417.91381
TATAACG1753.227322E-617.7333052
CGCGATC2359.558789E-917.60753635
ACGCAGA101400.017.3767347
CGCAGAG101650.017.3000768
GTCGTAC1200.001463714217.2740211
CCGATCG1402.0923762E-417.2407139
Produced by FastQC (version 0.11.5)