FastQCFastQC Report
Wed 25 May 2016
SRR1033217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500858
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96950.3876669527018327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.035.298091
GTATCAA71000.027.1143991
GCGGTAT1502.6269845E-823.2082251
ATCAACG87850.022.1620453
TCAACGC89100.021.9239374
CAACGCA91150.021.4308595
TATCAAC90200.021.1472662
AACGCAG93850.020.8522116
ACGGACG900.005709502419.18217842
CGAGCCG1651.875731E-618.76979819
ACGCAGA107050.018.1846037
CGGTGCG950.00797812518.1017467
GTACATG62150.017.9803071
GAGTACT76400.017.91801812
CGCAGAG108700.017.8769328
TAACGAC1552.2379747E-517.8154754
AGAGTAC103200.017.79761711
TACATGG61800.017.3477212
AGTACTT78900.017.26308613
GCAGAGT114150.016.692999
Produced by FastQC (version 0.11.5)