FastQCFastQC Report
Wed 25 May 2016
SRR1033217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500858
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.038.498371
GTATCAA75000.026.6664371
ATCAACG89800.022.0791913
TCAACGC90450.021.9586454
TATCAAC92250.021.4928052
CAACGCA92450.021.4836085
AACGCAG96000.020.6891616
GACGCGT850.004134031520.28349310
GTACATG63550.019.0239281
ACGCAGA109900.018.0096747
TACATGG64000.017.995262
CGCAGAG110450.017.8575528
GTGGTAT21550.017.6316571
CTGTCGC4900.017.5928259
GAGTACT81400.017.45277412
AGAGTAC107200.017.27313211
GTCGTAC1402.0645189E-417.271011
TCTGTCG5050.017.0702658
TGCACCG2652.2992026E-916.9156685
ACATGGG66050.016.7058583
Produced by FastQC (version 0.11.5)