FastQCFastQC Report
Wed 25 May 2016
SRR1033216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972706
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37720.3877841814484541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.039.408211
GTCTCGC2450.031.2093371
GCTATAC1151.7516868E-929.9222893
GTATCAA31100.029.279851
TCTGTCG2450.028.0830468
GTGGTAT7600.026.9815161
TGGTATC7850.026.5566582
CTGTCGC3100.024.4179239
TCTCGCT3050.023.9227582
TCGCCCA3400.023.27657712
TGTCGCC3400.023.27657710
ATCAACG40700.022.8276923
TCAACGC41000.022.737534
TATCAAC41050.022.006552
CAACGCA42100.021.9800175
AACGCAG42850.021.7558636
GTCGCCC3750.021.10409511
GTCTCAC4950.021.0641541
TATCACG1004.132002E-420.8469772
GTACATG22950.020.2931791
Produced by FastQC (version 0.11.5)