FastQCFastQC Report
Wed 25 May 2016
SRR1033216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972706
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.042.940491
GTATCAA30400.029.6731761
TCCGAAC605.4415356E-428.7319663
TGGTATC6300.025.721952
GTCTCGC2300.025.5457211
TACCTGG6250.025.3760722
GTGGTAT6700.025.2765851
CTCGTAT902.135082E-422.9855739
TACCGCT902.135082E-422.985577
ATCAACG39650.022.6957173
GTACCTG6600.022.5175761
TATCAAC40800.022.2250212
TCAACGC40650.022.1373984
CAACGCA41200.021.8418735
AGCGTTC800.002909689421.54897537
ATTAGAC2101.0186341E-1021.3437463
AACGCAG42400.021.3050236
TCTGTCG3050.020.3478858
GTACATG22550.020.2313331
CTGTCGC3250.020.1565789
Produced by FastQC (version 0.11.5)