FastQCFastQC Report
Wed 25 May 2016
SRR1033215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113852
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN208220.40716860792999093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73850.035.4548231
GTATCAA126600.029.4436661
TAGACCG1101.1870306E-625.0099775
ATCAACG156300.024.0758253
TCAACGC158300.023.6354544
CAACGCA161700.023.223555
AACGCAG166300.022.6647746
TATCAAC164350.022.4434762
GTACATG192000.021.0443611
TACATGG190350.019.907562
GCGAACT7550.019.6684228
ACGCAGA192550.019.4689127
ACATGGG194500.019.3473073
CGAACTA7350.019.26315329
CGCAGAG195550.019.029728
GTGGTAT34050.018.8923261
GAGTACT108150.017.87409412
AGAGTAC196600.017.6881311
GCAGAGT208450.017.3428069
AGTACTT111900.017.08965713
Produced by FastQC (version 0.11.5)