FastQCFastQC Report
Wed 25 May 2016
SRR1033215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113852
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76650.035.3856121
GTATCAA125100.027.3982961
ATCAACG155900.021.5638643
TCAACGC157100.021.3552534
CAACGCA160300.020.9719685
GTACATG196450.020.5779931
TATCAAC165600.020.4465122
AACGCAG166750.020.2229886
GCGAACT6900.019.98922528
GTGGTAT36650.019.7034151
TACATGG192700.019.7003382
CGAACTA7350.018.76539429
ACATGGG195550.018.6197643
AGCGAAC8900.017.43442227
ACGCAGA195550.017.1388137
TATAACG3054.7293724E-1116.9574092
CTAGCGG2451.6008926E-816.88885529
CGCAGAG198000.016.8222568
CGTATGG2502.0549123E-816.5504331
GAGTACT110450.016.29585312
Produced by FastQC (version 0.11.5)