FastQCFastQC Report
Wed 25 May 2016
SRR1033214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646666
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102650.38784644530137163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45600.035.651951
GTATCAA72400.025.7725541
ATCAACG88200.021.5354713
TCAACGC89200.021.17594
CAACGCA90800.020.7648625
TATCAAC89850.020.3007032
AACGCAG94250.020.0416566
GTACATG66550.019.6684911
ACGTCGC1501.7352733E-518.3502246
TACATGG67850.018.1614842
GAGTACT70400.018.0358912
ATACCGC2105.2030373E-818.0225416
AGAGTAC98650.017.78922311
ACGCAGA106300.017.7701037
ACATGGG69550.017.7615643
TAACGGC1753.1995296E-617.74949836
CGCAGAG107600.017.4917878
AGTACTT72700.017.27662313
TCTGTCG4900.016.8528868
GTACTTT75450.016.51418114
Produced by FastQC (version 0.11.5)