FastQCFastQC Report
Wed 25 May 2016
SRR1033214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646666
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.033.4228671
GTATCAA72000.026.7761861
ATCAACG89000.021.5024363
TCAACGC89450.021.432814
CAACGCA90800.021.114155
TATCAAC91750.020.8203662
AACGCAG92350.020.6477576
ACGCAGA106000.017.8912837
GAGTACT74050.017.88129812
AGAGTAC101250.017.67523611
GTACATG64650.017.6356891
CGCAGAG108050.017.5837488
AGTACTT76250.017.410613
TACATGG65350.016.990062
GTACTTT79650.016.75398314
GTGGTAT23900.016.4798771
CAGAGTA114350.016.10267310
GCAGAGT117350.015.955479
ACATGGG67550.015.6710353
TAGACTG8050.015.4202455
Produced by FastQC (version 0.11.5)