FastQCFastQC Report
Wed 25 May 2016
SRR1033213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413142
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95690.39653696301336594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1653.6379788E-1227.0868285
TACGAAC851.4373336E-424.36939663
GGTATCA94300.024.18321
TAGTGCG953.1342203E-421.7196649
GTATCAA130800.021.6872311
CCGTCGT1004.4316155E-420.633689
CGTCCTA850.004188629320.23753514
GGACCGT1601.4087236E-619.3396426
GAGTACT90600.019.16852212
CGAACGC1108.1983296E-418.83247465
AACGCAG154000.018.5973116
TCAACGC156650.018.4790844
ATCAACG157150.018.4698163
AGTACTT92000.018.46526313
TACATGG129000.018.4287782
CAACGCA156350.018.4265865
TATCAAC154000.018.3484762
GTACATG131800.018.2257021
ACGCTAT1150.001135033117.94195713
ACATGGG131850.017.6058963
Produced by FastQC (version 0.11.5)