FastQCFastQC Report
Wed 25 May 2016
SRR1033213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413142
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9450.025.1747839
GGTATCA98150.023.617631
TCTCGTA9750.022.63204238
TATGCCG10850.022.5619743
ATCTCGT10150.022.41952137
ATGCCGT11050.022.15360844
TCGTACG800.002910854521.5490632
CGACGGA850.00413637420.28147318
GTATCAA131500.020.2287521
TGCCGTC12650.020.16924345
GCCGTCT13050.019.28682746
ACACCGA1801.9966501E-719.1547246
TCGTATG12750.018.92937540
CTACATC12500.018.75630633
CCGTCTT13650.018.69164347
CGTATGC13150.018.35357541
CGTCTTC13700.018.12008948
TTACGAC1351.5853923E-417.8777418
TACATGG129850.017.099852
CGGACAA5250.017.07542228
Produced by FastQC (version 0.11.5)