FastQCFastQC Report
Wed 25 May 2016
SRR1033212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096002
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122360.3952193829332151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44900.040.6009031
GTATCAA80900.030.3173581
GTGGTAT19350.024.631471
ATCAACG103150.024.1731193
TCAACGC104700.023.8113884
CAACGCA105700.023.4559825
TATCAAC105700.023.1015722
AACGCAG110700.022.4911946
TGGTATC19750.022.1913052
GTCCGAT1357.0133246E-620.3817411
GTACATG79000.020.1251851
ACGCAGA124550.019.8806937
CGCAGAG126350.019.5430348
TACATGG76900.019.5405522
GAGTACT93650.018.76714312
ACATGGG80050.018.5173933
AGAGTAC127050.018.27318211
CAGAGTA132450.017.81382810
AGTACTT98700.017.7023813
GCAGAGT136250.017.6704089
Produced by FastQC (version 0.11.5)