FastQCFastQC Report
Wed 25 May 2016
SRR1033212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096002
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.041.157831
GTATCAA81700.028.2423171
TGGTATC20150.026.8678822
GTGGTAT21800.026.7778761
GACGCGT700.00133824.63132710
ATCAACG98300.023.0122533
TATACCG1052.2909528E-522.9888675
TCGCGTA750.001999401522.98886746
TCAACGC99000.022.8843734
CAACGCA101550.022.3776425
TATCAAC103950.022.026852
AACGCAG104500.021.713286
GTACATG79600.021.002851
TACATGG78250.020.359472
ACATGGG79500.019.345353
ACGCAGA122950.018.2866827
CGCAGAG123550.018.1141438
CGAACGT1150.001114294317.9915854
GAGTACT90450.017.34705412
AGAGTAC126800.016.80707411
Produced by FastQC (version 0.11.5)