FastQCFastQC Report
Wed 25 May 2016
SRR1033211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462945
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97370.3953397254100275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.039.502091
GTATCAA63750.029.907991
ATCAACG79300.024.4230273
TCAACGC80250.024.0447374
CAACGCA82450.023.4865915
TATCAAC81750.023.1902752
GTACGGT750.002028639722.9313036
AACGCAG85900.022.704396
GTGGTAT14900.021.4826771
TATAACG1506.780501E-720.8712482
TAGGTCG850.004194482720.2326745
GTACATG65200.019.9043541
TACCTGG16100.019.8773782
ACGCAGA98000.019.7962027
TGGTATC16250.019.6938952
CGCAGAG98500.019.625878
GTACCTG17300.019.5079881
CGACCGT1258.678031E-519.33968221
TACATGG65150.019.3281962
TAACGGC1501.6893095E-518.40742536
Produced by FastQC (version 0.11.5)