FastQCFastQC Report
Wed 25 May 2016
SRR1033211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462945
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37850.037.981551
GTATCAA65650.028.6358051
GTGGTAT17600.026.507351
TGGTATC17400.025.3655242
TATAACG1803.45608E-1022.9875072
ATCAACG80800.022.6603343
TCAACGC80600.022.5882234
CAACGCA82800.022.0296965
TATCAAC84050.021.907192
ATAACGC1906.8030204E-1021.777643
AACGCAG86600.021.1426666
TACCTGG13750.020.0618252
GTACATG63400.019.6226861
TCTGTCG5150.018.7470938
CTGTCGC5900.018.7017029
ACGCAGA98950.018.4341477
CGCAGAG98600.018.429648
TACATGG63800.018.3755932
TAACGCA3350.017.4979534
GTACCTG16400.017.2788661
Produced by FastQC (version 0.11.5)