FastQCFastQC Report
Wed 25 May 2016
SRR1033210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3781291
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149760.39605520971541197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50300.037.200371
GTATCAA83550.028.59851
ATCAACG103950.022.9628773
TCAACGC104500.022.8721984
CAACGCA105850.022.613285
AACGCAG110150.021.8241756
TATCAAC109450.021.4462282
GTGGTAT22750.020.7917231
GTACATG98950.020.5623631
TACATGG100200.019.7819942
ACGCAGA128200.018.6448677
ACATGGG102150.018.5525913
CGCAGAG129500.018.4048258
TGGTATC24400.017.8148882
GAGTACT89500.017.29364812
AGAGTAC131100.016.68597411
GAGTACA72750.016.5893541
GCAGAGT141450.016.1702169
CAGAGTA139200.016.15978810
AGTACTT95650.016.00213613
Produced by FastQC (version 0.11.5)