FastQCFastQC Report
Wed 25 May 2016
SRR1033210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3781291
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54200.037.2360461
GACGCGT751.6772956E-632.18343710
GTATCAA86300.026.3891031
ACGCGTT1103.2824697E-521.94325311
GTGGTAT26900.021.8396991
CGCGTTG1304.948746E-621.21984912
TCAACGC104850.021.1790924
ATCAACG105000.021.1488363
GTACATG101350.021.0383381
CAACGCA107200.020.7148115
TATCAAC109200.020.4617272
TACATGG99700.020.1979642
AACGCAG111900.019.9066476
TGGTATC27550.019.7754042
ACATGGG103000.018.680433
CGAACTA3003.6379788E-1117.24158329
GCGAACT3400.017.24158328
ACGCAGA134250.016.5668957
CGCAGAG134450.016.4396618
TATACTG16450.016.1404175
Produced by FastQC (version 0.11.5)