FastQCFastQC Report
Wed 25 May 2016
SRR1033209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546242
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139460.39326137358928126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.035.9188461
GTATCAA71050.028.4858071
ATCAACG86600.023.9036083
TCAACGC86850.023.793864
CAACGCA89300.023.0640535
AACGCAG92650.022.2684866
GTACATG89600.022.1226181
TATCAAC92300.021.4720922
TACATGG89000.021.2525022
ACATGGG93450.020.0172523
ACGCAGA106400.019.3589767
CGCAGAG109950.018.7339238
GAGTACA64050.017.971261
TATACCG1552.3140443E-517.7467385
TACACCG3700.017.6568055
AGAGTAC110200.017.5995211
GAGTACT78850.017.3137912
GTACCGT1804.3354376E-617.1931276
GCAGAGT120050.016.8716099
CATGGGG58600.016.6640664
Produced by FastQC (version 0.11.5)