FastQCFastQC Report
Wed 25 May 2016
SRR1033209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546242
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46650.036.5116731
GTATCAA74250.024.987011
GTACATG92900.022.1649631
TACATGG93150.021.321512
TACACCG3900.021.2190045
ATCAACG88950.020.5063363
TCAACGC89800.020.3122334
CAACGCA92400.019.8153115
ACATGGG96000.019.6113
TATCAAC94700.019.3340512
AACGCAG96650.019.1580286
GTGGTAT23700.018.3678651
TAGCGCG1351.584821E-417.8789754
GAGTACA65550.017.393131
ACACCGT5200.017.2404426
CTACACT11050.016.8503864
TGGTATC24050.016.6311112
CGAACTA2502.053821E-816.55082529
ACGCAGA115650.015.9211347
AGTACAT64750.015.8691932
Produced by FastQC (version 0.11.5)