FastQCFastQC Report
Wed 25 May 2016
SRR1033208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027778
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157240.390388943978541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.036.370121
GTATCAA72550.029.6652261
ATCAACG87800.024.486163
TCAACGC88200.024.3717654
CAACGCA90100.023.819655
TATCAAC91450.023.07372
AACGCAG93750.023.0393036
GTACATG101150.022.9617521
TACATGG98900.022.0737252
ACATGGG104750.020.8523453
ACGCAGA110600.019.281437
CGCAGAG111900.018.9959538
GAGTACT79000.017.89530412
AGAGTAC117800.017.37384811
GTACGAC1855.6092977E-616.734243
CAGAGTA123900.016.68505110
AGTACTT85450.016.54493113
GCAGAGT126300.016.5314039
GTGGTAT22900.016.3976921
TATACTG17100.016.0904965
Produced by FastQC (version 0.11.5)