FastQCFastQC Report
Wed 25 May 2016
SRR1033208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027778
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.036.7239651
GTATCAA75150.026.1599981
GTGGTAT22000.021.672591
ATCAACG91100.021.3477293
TCAACGC91450.021.2660264
CAACGCA93550.020.7886495
AACGCAG97800.019.9205136
GTACATG104600.019.917741
TATCAAC99450.019.7633722
TACATGG103850.019.2580572
GTACCGT2759.094947E-1218.80836
TGGTATC23600.018.555932
ACATGGG107800.017.656773
ACGCAGA115950.016.7130817
TTGCGCG2301.433873E-716.49133518
ACCGTAC6950.016.42243669
CGAACTA2101.0043586E-616.42014929
TAGACTG13500.016.3469665
CGCAGAG118100.016.321238
GAGTACA77750.016.0865381
Produced by FastQC (version 0.11.5)