FastQCFastQC Report
Wed 25 May 2016
SRR1033207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235400
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124500.38480558818075045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38500.025.5557581
GGTATCA25200.025.109261
GTACATG85900.024.0007381
TACATGG83400.023.1346382
TCAACGC46100.021.8592034
ACATGGG89450.021.3436163
CAACGCA47600.021.3152035
ATCAACG47850.021.13593
GAGTACA58500.020.2656961
AACGCAG51350.019.8264926
AGTACAT56550.018.9959772
CGAACTA3100.018.9373829
TATCAAC53400.018.0983642
CTACACT11900.017.6299274
GTACTAG3750.017.615111
CATGGGG60800.017.479244
TACACTG16150.017.036955
ACGCAGA62250.016.1901137
CGCAGAG65100.015.4810898
GTTACAC6500.015.3475853
Produced by FastQC (version 0.11.5)