FastQCFastQC Report
Wed 25 May 2016
SRR1033207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235400
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.026.9779431
GTATCAA45050.024.1565511
GTACATG93600.024.102191
TACATGG93050.023.2721312
GAGTACA63650.021.9824371
ACATGGG98550.021.3785113
ATCAACG50300.021.18283
TCAACGC50100.021.129714
CAACGCA51400.020.6623865
AGTACAT61900.020.4998192
AACGCAG54050.019.7131336
TATCAAC57450.018.7265382
CGAACTA3150.018.60935229
CATGGGG65150.017.7305954
CCGATCG1704.9875438E-516.2268418
TACGCGA1300.00253182215.91478444
TACACCG4550.015.9147845
ACGCAGA68200.015.7242147
TTACGCG1554.545244E-415.57253243
CGCAGAG70350.015.1946428
Produced by FastQC (version 0.11.5)