FastQCFastQC Report
Wed 25 May 2016
SRR1033206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330602
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87690.3762547187379055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.041.150671
GTATCAA61500.030.033481
GTGGTAT14750.025.233531
TCAACGC75700.024.539284
ATCAACG76050.024.5199853
CAACGCA77050.024.1539735
TGGTATC15200.023.7968332
TATCAAC78050.023.3054872
AACGCAG81050.022.9194776
ACGCAGA91500.020.1895437
CGCAGAG93100.019.7686678
GTACATG65750.019.3629511
AGAGTAC92800.018.83289711
TACATGG64100.018.2852652
GAGTACT65600.018.25058612
CAGAGTA96350.018.24612210
GCAGAGT99700.017.94369
ACATGGG67000.017.66463
ACACCGT2752.055458E-1017.5129496
AGTACTT68750.017.01404813
Produced by FastQC (version 0.11.5)