FastQCFastQC Report
Wed 25 May 2016
SRR1033206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330602
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.041.766811
TAGTGCG703.865387E-529.5571777
GTGGTAT15200.029.320221
GTATCAA63900.026.1722662
TGGTATC17800.024.021612
ATCAACG80550.021.019214
TCAACGC80800.020.8688225
TATCAAC82550.020.468193
CAACGCA82950.020.2452136
AACGCAG85400.019.6644047
GTACATG67050.018.033931
TACATGG67700.017.1649152
ACGCAGA100300.016.6744148
CGCAGAG101500.016.511259
ATACCGC1907.057457E-616.3342296
ACATGGG70100.015.9377663
CAATCGA1300.002531243715.91506138
AGAGTAC102100.015.56986812
GAGTACT72550.015.54247112
GTATAGG5350.015.4981031
Produced by FastQC (version 0.11.5)