FastQCFastQC Report
Wed 25 May 2016
SRR1033205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851386
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31400.3688103868280663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.10101176199749586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.035.1959651
GTACCTG5800.032.3506851
TACCTGG5700.030.4780372
ATAACGC703.936005E-529.475643
GTATCAA31750.026.4843521
ATCGTAC1208.79445E-825.78814111
GTCTCGC2800.024.8193111
TATAACG851.3752075E-424.5258562
TCTGTCG2950.024.4754378
TCTCGCT2900.023.9620442
CTGTCGC3350.022.5806629
CGCTCTG3450.021.9248545
GCTATAC953.1326586E-421.7188913
TACCGTG953.1364095E-421.7150485
TAACGCA800.002956929621.4888524
ATCAACG39350.021.3233363
TCAACGC39950.021.085434
TCGCTCT3950.020.8904294
TATCAAC39350.020.7498512
CAACGCA40550.020.6886485
Produced by FastQC (version 0.11.5)