FastQCFastQC Report
Wed 25 May 2016
SRR1033205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851386
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG758.767529E-1041.377132
GGTATCA20600.037.0635221
ATAACGC905.2150426E-934.4809383
TATCACG751.6757749E-632.1822132
TACCTGG6350.029.3223742
GTACCTG6550.029.0096861
TAACGCA1103.680543E-828.211684
GTATCAA30900.024.6611252
GTATAAC1551.4988473E-924.5178621
TCACGCA1052.2896398E-522.9872954
CGGACAA902.1336955E-422.98729328
GTGGTAT8950.022.7745911
TCTGTCG2350.022.0091118
TCGCTCT2401.8189894E-1221.550594
GTCTCGC2151.364242E-1020.889431
CTGTCGC2501.8189894E-1220.6885669
TGGTATC9050.020.5742632
ATCAACG37800.020.341934
ACGGACA850.00413251820.28290727
TCAACGC37750.020.2775345
Produced by FastQC (version 0.11.5)