FastQCFastQC Report
Wed 25 May 2016
SRR1033204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959191
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77800.39710268166809665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.033.51251
GTATCAA54950.029.7940541
ATCAACG69200.024.3585993
TCAACGC70600.023.871284
CAACGCA71750.023.5366085
TATCAAC70600.023.2351842
AACGCAG74950.022.5328666
GTGGTAT15850.022.3690361
CGAACTA1004.3127182E-420.71672629
GTACATG55250.020.5727251
TGGTATC16750.019.9188942
ACGCAGA84700.019.7370267
CGCAGAG85500.019.5523538
TACATGG55600.019.5024242
GAGTACT60400.019.30745712
AGAGTAC83950.019.09485411
AGTACTT61450.018.86510313
ACATGGG57950.018.8177683
TAGACTG4950.018.7604335
CAGAGTA89200.018.2409510
Produced by FastQC (version 0.11.5)