FastQCFastQC Report
Wed 25 May 2016
SRR1033204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959191
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.038.5389941
GTATCAA57850.028.1926691
TCAACGC69500.022.87144
ATCAACG69550.022.8549583
TATCAAC72200.022.3503992
CAACGCA71100.022.3082185
GTGGTAT15400.021.7644941
AACGCAG74100.021.4981166
TGGTATC15850.021.3193242
ATAACGC1701.09230314E-720.2827913
GTACATG57500.019.0497361
ACTGCGT1501.7030767E-518.389738
CGCAGAG87050.018.2603368
ACGCAGA87100.018.2498557
TCTGTCG4200.018.0613428
TCGTATG1150.001114771417.98995437
CGGACTA1150.001114771417.98995434
TACATGG58350.017.964262
AGAGTAC86600.017.51953111
GAGTACT62750.017.47435212
Produced by FastQC (version 0.11.5)