FastQCFastQC Report
Wed 25 May 2016
SRR1033203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436951
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95730.3928269382519386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38900.037.7098961
GTATCAA66600.029.332871
GTGGTAT18100.025.3505761
ATCAACG81200.024.3960233
TCAACGC82350.024.179634
TGGTATC18300.023.930322
CAACGCA84450.023.5376385
TATCAAC84200.023.0743122
AACGCAG86550.023.007696
ACGCAGA98350.020.178137
CGCAGAG98950.019.986268
GAGTACT63300.019.94035512
AGAGTAC96450.019.07752611
GTACATG72000.018.9254441
AGTACTT67050.018.82473413
TGAGTCG1301.21094665E-418.5177975
GTACTTT70200.018.08100114
CAGAGTA104900.017.90142810
GCAGAGT106900.017.8882399
TACATGG71800.017.8620382
Produced by FastQC (version 0.11.5)