FastQCFastQC Report
Wed 25 May 2016
SRR1033203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436951
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.037.3198361
GTATCAA68750.028.4422151
GTGGTAT19100.025.5038281
TGGTATC20450.022.2576712
ATCAACG87700.022.097133
TCAACGC87900.021.9291614
TATCAAC91600.021.306892
CAACGCA90950.021.2316825
TATAACG1304.947209E-621.220022
AACGCAG93600.020.6305716
ACGCAGA107750.017.7933047
GAGTACT72750.017.72746712
CGCAGAG109350.017.4068188
GTACATG75100.017.1128621
AGAGTAC108400.016.9553211
AGTACTT75750.016.93434113
GTACTTT76100.016.81114614
TACACCG2702.9449438E-916.60275
TATGCCG1907.0593596E-616.3338343
GCAGAGT115750.016.2361689
Produced by FastQC (version 0.11.5)