FastQCFastQC Report
Wed 25 May 2016
SRR1033202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363617
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48400.3549383734582364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.034.3094751
GTATCAA27150.030.4641571
TGCGTAT700.001326731324.66587350
GTGGTAT7500.023.6314811
ATCAACG35000.023.578873
TCAACGC35150.023.4765194
CAACGCA35850.023.018125
TATCAAC35250.022.9675222
AACGCAG36850.022.3934786
GTACATG39000.021.2968461
TATGTCG3400.020.2254815
TACATGG40850.019.7339152
ACATGGG41100.019.1590273
TGGTATC8950.019.0234992
CGGACAA2406.020855E-1018.70495228
ACGCAGA43750.018.6266217
CGCAGAG44000.018.4433218
CACGTAC2651.1459633E-1018.23668761
CTAGTAC1150.001135329317.9404473
GAGTACT28200.017.92586312
Produced by FastQC (version 0.11.5)