FastQCFastQC Report
Wed 25 May 2016
SRR1033202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363617
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.038.953831
GTGGTAT8650.028.7484021
GTATCAA34400.027.5099261
TCGAACG851.4481695E-424.34133353
TGGTATC10200.022.9890372
CGAACGT902.1335151E-422.98903554
ATCAACG41500.022.7674543
TATCAAC42350.022.3919182
TCAACGC42450.022.3391694
CAACGCA43250.021.9259595
AACGCAG45400.020.8876156
CGTATAC1004.349347E-420.6901323
CGATCGC1056.0431525E-419.7048919
ATTCGGT1258.774612E-519.31079128
TCGCACG1108.2642434E-418.80921222
ACGCAGA53600.017.6277877
GGCTAGA3003.6379788E-1117.2690051
ACCGTCC1200.001481692917.2417778
CGCAGAG55200.017.1793088
GAGTACT33200.016.72244812
Produced by FastQC (version 0.11.5)