FastQCFastQC Report
Wed 25 May 2016
SRR1033201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537773
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18490.3438253686964574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.00714087452.1414682
GATACGT200.007449746651.5860988
ATCGCGC200.007449746651.5860988
GGTATCA5300.034.770831
CAATCGA400.00259020434.51326859
GTATCAA9550.031.676041
GCGTAAG500.00744174227.8166641
TCGGACT500.00784115327.520313
TCAGCGG658.8079483E-426.456883
ATCAACG12850.023.8214873
TCAACGC12900.023.720294
TATCAAC12950.023.3529362
CAACGCA13500.022.6660545
GTGGTAT2800.022.3526761
CCGTCTG800.002948051821.4982249
GCTAGCC800.002949650921.4962163
GACCGGG800.002951250421.4942077
AACGCAG14250.021.4751116
GGCTTAG1206.0063285E-520.2829861
CCGAAAT900.005699191719.1848347
Produced by FastQC (version 0.11.5)