FastQCFastQC Report
Wed 25 May 2016
SRR1033201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537773
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA200.0073717951.7235732
GGTATCA7300.036.4497761
GTATCAA12900.029.1987191
CCGCCGA500.007652628227.65791161
GTGGTAT3650.024.6154331
TCAACGC16000.023.4911214
ATCAACG16050.023.417943
CAACGCA16550.022.7104515
AACGCAG16600.022.6420446
TGGTATC3500.021.6746392
TATCAAC17200.021.6517282
GCAACGT800.00285777121.61378768
AATACCG800.00290604921.5514895
TAAGACT2301.4551915E-1120.9892754
CAACGTT850.004061112620.34238869
ACGCAGA19800.018.9827257
GTAGGGG1108.257251E-418.808573
CGCAGAG20250.018.5608848
TCTGTAC2458.039933E-1018.2967723
TAGACAG1702.4392612E-618.2553795
Produced by FastQC (version 0.11.5)