FastQCFastQC Report
Wed 25 May 2016
SRR1033200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964476
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72220.3676298412401068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.037.3190571
GTATCAA47100.032.8730351
GTGGTAT11900.026.8993381
ATCAACG59150.026.5158143
TCAACGC59750.026.1859534
TATCAAC60050.025.7232422
CAACGCA61800.025.3173265
AACGCAG63600.024.7636366
TGGTATC13550.022.5943132
ACGCAGA73350.021.1443127
CGCAGAG74400.020.984578
GTACATG61700.019.9062861
TACATGG59750.019.5639762
AGAGTAC74400.019.55270211
GAGTACT51100.019.38255112
GCAGAGT79050.019.1421249
CAGAGTA77650.019.08864610
AGTACTT51900.018.88547713
AGCGCGA1108.192763E-418.83412247
ACATGGG60950.018.791673
Produced by FastQC (version 0.11.5)