FastQCFastQC Report
Wed 25 May 2016
SRR1033200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964476
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27850.038.090451
GTATCAA49150.029.4573331
GTGGTAT13350.023.8127461
ATCAACG60800.023.4229743
TCAACGC62250.022.8219874
CAACGCA63250.022.4611645
TATCAAC64200.022.2362162
AACGCAG65400.021.6700366
TGGTATC14050.020.8611452
TGTCGCG1056.0449593E-419.70464323
GTACATG59950.019.53951
ACGCAGA77200.018.3577777
TACATGG60150.018.1726882
CGCAGAG79150.017.7748038
CGAACTA1402.0913285E-417.24156229
ACATGGG61400.017.1849653
AGAGTAC78100.016.86583711
TAGACAG7450.016.6625635
GCAGAGT84250.016.2895369
CAGAGTA82400.016.27863310
Produced by FastQC (version 0.11.5)