FastQCFastQC Report
Wed 25 May 2016
SRR1033199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAAT70.4820936639118457No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGC70.4820936639118457No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCAC60.4132231404958678No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGA50.34435261707988984No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGAC50.34435261707988984No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTT50.34435261707988984No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTC40.27548209366391185No Hit
GGCGATTTCCCCCATGTCTGGTTTGTCTGCCATTTTCTTAAAACAATCCGTGCAGTCTCGTTCCGAGCCCACGAG40.27548209366391185No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGC40.27548209366391185No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGC40.27548209366391185No Hit
GGACAGAGGTGTCACCTTGCAGGAAGGAAGCAGAGCCATCTGGCCGCGACAGCAGGTTCTGTTCGCAGGCAAAGT30.2066115702479339No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCT30.2066115702479339No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAG30.2066115702479339No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG30.2066115702479339No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGA30.2066115702479339No Hit
TTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAAC30.2066115702479339No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA30.2066115702479339No Hit
AGGTGGGAGATGCCATCCCAGCAGTGGAGGTGTTTGAAGGGGAGCCAGGGAACAAGGTGAACCTGGCAGAGCTGT20.13774104683195593No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCC20.13774104683195593No Hit
ATCTGATTCAGCAGCTCCAGCAGGATCCTCTGAACCTCCCTGTCGGCCCCTGTCTGAGCATCGAATCTCTTGGTG20.13774104683195593No Hit
GCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCA20.13774104683195593No Hit
GTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCG20.13774104683195593No Hit
GGACAAGTTCCAGGGCGAGGATATGGAATTCTGCCCTGATAGGAAATCAGCTGATGATTGGGCCCTTCTTTGTGG20.13774104683195593No Hit
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGC20.13774104683195593No Hit
CTTCCTCCACATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACT20.13774104683195593No Hit
GTTCAAGACCAGCCAGGGCAACATGGAGAAACCCCGTCTCTACAAAAAAATTAGCCAGGCATGGTGATGTGTGCC20.13774104683195593No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCG20.13774104683195593No Hit
CTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCCA20.13774104683195593No Hit
GTTTGTGGCCATGATTACCTTGACATTGACATTCTGATCAAATCCATCCATCTGATTCAGCAGCTCCAGCAGGAT20.13774104683195593No Hit
CCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAAT20.13774104683195593No Hit
GTTCAAGGGCAAGAAGGGTGTGCTGTTTGGAGTTCCTGGGGCCTTCACCCCTGGATGTTCCAAGACACACCTGCC20.13774104683195593No Hit
TTCCAAGACACACCTGCCAGGGTTTGTGGAGCAGGCTGAGGCTCTGAAGGCCAAGGGAGTCCAGGTGGTGGCCTG20.13774104683195593No Hit
ATATACTCGTCGGTGGGGCCGGCGGTCAGTCTGCGGCAGCGGCAGCAAGACGGTGCAGTGAAGGAGAGTGGGCGT20.13774104683195593No Hit
GATCCAGGGTGTCTGCTCTGTTTGTGGCCATGATTACCTTGACATTGACATTCTGATCAAATCCATCCATCTGAT20.13774104683195593No Hit
CGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAG20.13774104683195593No Hit
GAAGAGAGCCCGCATGGGCACACCTGCATCCACCAATGCCATGCAGGCGGCATTCAGACAACAGGCCAGGAGAGA20.13774104683195593No Hit
GGTGTTCTTCTCCTGCGTCTCCGTTTTCTTCAGCTTGGCCTTATCGAAGCTGGCGATTTCCCCCATGTCTGGTTT20.13774104683195593No Hit
TTGTTAAACAGAATAAAATGGAAATTCACCTTGTCATCTACCCGACATTGGCCTTCCTGTGCCACGGCATCATGG20.13774104683195593No Hit
AGTGTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCATTTTCCGGAGGGAGGCCCCATAGCGGGTCCCGTA20.13774104683195593No Hit
CGTTGGGACAGAAATGCTTGACTTCTGGGGTCCACTTTTCTGGGATGTTTTCTAAACTATCAGGGCTGTCGATGG20.13774104683195593No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)