FastQCFastQC Report
Wed 25 May 2016
SRR1033198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287811
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78470.34299161950003737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46400.028.1228181
GGTATCA28300.027.9116131
ATCAACG55950.023.6132093
TCAACGC56500.023.3802644
CAACGCA57950.022.7957555
GTACATG77650.022.675381
AACGCAG60100.022.095236
TATCAAC58450.022.0259062
TACATGG78450.021.555592
ACATGGG80150.021.1195893
CGAACTA2051.724402E-920.2128829
TACACCG3100.019.975015
GAGTACA54850.018.8419281
ACGCAGA71250.018.6375247
CGCAGAG73450.018.0324468
AGTACAT52000.017.999712
GAGTACT40400.017.96864912
AGAGTAC69950.017.90309111
GTACTTT40450.017.86530914
AGTACTT40150.017.73777413
Produced by FastQC (version 0.11.5)