FastQCFastQC Report
Wed 25 May 2016
SRR1033198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287811
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.028.8657681
GTATCAA47750.024.8830971
GTACATG81450.022.0087641
TACATGG81050.021.4008662
ACATGGG82400.020.2969573
TACACCG3250.020.1598225
ATCAACG59900.019.688643
TCAACGC60000.019.6558234
CAACGCA61200.019.2704185
AACGCAG63150.018.7845826
TATCAAC63400.018.6017272
TAAACCG2053.848072E-818.5035575
AAGCGCG1501.7019369E-518.39141746
GAGTACA56050.018.3636931
ATATCGT950.00785787318.1494246
ACACCGT4000.018.104056
CGCGAAC1150.001114163517.99160228
TCCGCGA1753.2249354E-617.7345826
ACCGTCC2006.024111E-717.2419538
AGTACAT55100.017.0229072
Produced by FastQC (version 0.11.5)