FastQCFastQC Report
Wed 25 May 2016
SRR1033197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4069312
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51600.035.2427641
GGTATCA34500.034.9585341
TCAACGC53900.032.33534
ATCAACG54350.032.160343
CAACGCA56600.031.0414225
AACGCAG60050.029.0122786
GTACATG129800.027.3308181
TACATGG129350.026.0973852
TATCAAC69550.025.7553122
ACGCAGA71850.024.1168467
ACATGGG137950.023.4338683
CGCAGAG75700.022.4560628
GAGTACA94250.020.8442861
CATGGGG90450.020.6712284
TATACCG2601.69548E-819.8749395
GCAGAGT89950.018.063259
AGTACAT98600.018.0241132
GTCTACG3501.4661055E-917.4987771
TATACTG26050.017.1317735
AGAGTAC76600.015.72459810-11
Produced by FastQC (version 0.11.5)