FastQCFastQC Report
Wed 25 May 2016
SRR1033197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4069312
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74530.18315135334916566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59120.1452825440762468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57200.047.9646071
GTATCAA103300.044.645131
TCAACGC125000.036.4424974
ATCAACG126900.035.933913
CAACGCA128950.035.3626485
TATCAAC135150.033.9838752
AACGCAG134300.033.9447566
ACGCAGA154150.029.2684237
CGCAGAG159150.028.2592668
GTACATG123450.025.5787771
GCAGAGT177350.025.0147659
TACATGG125950.023.962452
TATACCG2202.4501787E-923.5052245
ACATGGG130650.022.0569523
AGAGTAC161850.020.74651310-11
GAGTACT117600.020.66040412-13
CAGAGTA173250.019.99170710-11
GTGGTAT31350.019.9646841
CATGGGG86600.019.542454
GAGTACA91100.019.371411
Produced by FastQC (version 0.11.5)