##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033197_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4069312
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78139867378073	34.0	31.0	34.0	31.0	34.0
2	32.874764087885126	34.0	31.0	34.0	31.0	34.0
3	32.97046675211928	34.0	31.0	34.0	31.0	34.0
4	36.3793061333218	37.0	37.0	37.0	35.0	37.0
5	36.32105574603275	37.0	37.0	37.0	35.0	37.0
6	36.40071294607049	37.0	37.0	37.0	35.0	37.0
7	36.3733446833273	37.0	37.0	37.0	35.0	37.0
8	36.37503243791579	37.0	37.0	37.0	35.0	37.0
9	38.20334469315698	39.0	39.0	39.0	37.0	39.0
10-11	38.178426107410786	39.0	39.0	39.0	37.0	39.0
12-13	38.14188590110564	39.0	39.0	39.0	37.0	39.0
14-15	39.6806435584197	41.0	40.0	41.0	37.0	41.0
16-17	39.695712936240824	41.0	40.0	41.0	37.0	41.0
18-19	39.706126244436405	41.0	40.0	41.0	37.0	41.0
20-21	39.700482538571634	41.0	40.0	41.0	37.0	41.0
22-23	39.6320450484013	41.0	40.0	41.0	37.0	41.0
24-25	39.581300966846484	41.0	40.0	41.0	37.0	41.0
26-27	39.47950439779501	41.0	39.5	41.0	36.5	41.0
28-29	39.390079944717925	41.0	39.0	41.0	36.0	41.0
30-31	39.29833458333988	41.0	39.0	41.0	36.0	41.0
32-33	39.33522411159429	41.0	39.0	41.0	36.0	41.0
34-35	39.278570308691	41.0	39.0	41.0	35.0	41.0
36-37	39.191695058034384	41.0	39.0	41.0	35.0	41.0
38-39	39.10526337130207	41.0	39.0	41.0	35.0	41.0
40-41	38.987491128721516	41.0	39.0	41.0	35.0	41.0
42-43	38.870785651235394	41.0	39.0	41.0	35.0	41.0
44-45	38.720733381957444	41.0	39.0	41.0	35.0	41.0
46-47	38.503130996099586	41.0	38.5	41.0	35.0	41.0
48-49	38.311089196404694	41.0	38.0	41.0	35.0	41.0
50-51	38.167529425121494	40.5	38.0	41.0	34.0	41.0
52-53	38.02215521935895	40.0	38.0	41.0	34.0	41.0
54-55	37.878159625017695	40.0	37.0	41.0	34.0	41.0
56-57	37.704192993803375	40.0	37.0	41.0	34.0	41.0
58-59	37.50514964200337	40.0	36.5	41.0	33.0	41.0
60-61	37.30050644924744	40.0	36.0	41.0	33.0	41.0
62-63	37.05682680512086	39.0	35.5	41.0	33.0	41.0
64-65	36.801073006935816	39.0	35.0	41.0	33.0	41.0
66-67	36.51710338258654	38.5	35.0	41.0	32.5	41.0
68-69	36.197180751930546	37.5	35.0	40.5	32.0	41.0
70-71	35.86595363049085	37.0	35.0	40.0	32.0	41.0
72-73	35.517237926214555	36.5	35.0	39.0	31.0	41.0
74-75	35.18690960535835	36.0	35.0	39.0	31.0	41.0
76-77	34.85169962391677	35.5	35.0	38.5	31.0	40.5
78-79	34.52458794017269	35.0	35.0	37.0	31.0	39.0
80-81	34.19850444006259	35.0	35.0	37.0	31.0	39.0
82-83	33.889270348402874	35.0	34.0	36.5	30.5	39.0
84-85	33.6325968615825	35.0	34.0	36.0	30.0	37.0
86-87	33.412912674181776	35.0	34.0	36.0	30.0	37.0
88-89	33.20742376106821	35.0	34.0	35.5	30.0	37.0
90-91	33.02090992285674	35.0	34.0	35.0	30.0	36.0
92-93	32.869309971808505	35.0	34.0	35.0	30.0	36.0
94-95	32.730989784022455	35.0	34.0	35.0	29.5	36.0
96-97	32.61369821237595	35.0	34.0	35.0	29.5	36.0
98-99	32.51686206415236	35.0	34.0	35.0	29.0	35.5
100	32.42542695177956	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09387171644031156
1101	2	0.15816269325982546
1101	3	0.16222004758297714
1101	4	0.08912821335153609
1101	5	0.08998744768970113
1101	6	0.11189317548777922
1101	7	0.13647958450389552
1101	8	0.11775629942062693
1101	9	0.17670225095190517
1101	10-11	0.16269797570014077
1101	12-13	0.19080572342077318
1101	14-15	0.2287635687130276
1101	16-17	0.2275690164247166
1101	18-19	0.2592472706731712
1101	20-21	0.21880568095232178
1101	22-23	0.20362752065570078
1101	24-25	0.1852839703701079
1101	26-27	0.21676563507421776
1101	28-29	0.22508681609768644
1101	30-31	0.26039664575226595
1101	32-33	0.23137477884144175
1101	34-35	0.23481589491976962
1101	36-37	0.27671252168289584
1101	38-39	0.19342979541702476
1101	40-41	0.24529153901183065
1101	42-43	0.19612715145139248
1101	44-45	0.18724293312958906
1101	46-47	0.3008030256845231
1101	48-49	0.2738441898014372
1101	50-51	0.27717663769485057
1101	52-53	0.31128394663685555
1101	54-55	0.31793930501155643
1101	56-57	0.32761066853017695
1101	58-59	0.3363007492473713
1101	60-61	0.3660963621526321
1101	62-63	0.334057536772022
1101	64-65	0.32714231944497385
1101	66-67	0.33015972024239915
1101	68-69	0.36986414528875144
1101	70-71	0.36041270403956815
1101	72-73	0.42246281222442406
1101	74-75	0.3576995036903199
1101	76-77	0.31770860863348815
1101	78-79	0.30345847323381747
1101	80-81	0.23374145218476627
1101	82-83	0.2775973380193406
1101	84-85	0.30333539402231935
1101	86-87	0.25230346062632236
1101	88-89	0.2920504390413612
1101	90-91	0.3125533427925049
1101	92-93	0.3467304843568755
1101	94-95	0.3582709094554275
1101	96-97	0.397608904768731
1101	98-99	0.4047301330709061
1101	100	0.4433903863296109
1102	1	0.0907143017909462
1102	2	0.12451439354661176
1102	3	0.12980560640328775
1102	4	0.058615805264771836
1102	5	0.047999489052337196
1102	6	0.0904955754329464
1102	7	0.08327531244007247
1102	8	0.10886703473292414
1102	9	0.13847825905890687
1102	10-11	0.10193053900889737
1102	12-13	0.0973738966349913
1102	14-15	0.14144927684930764
1102	16-17	0.14173238257425425
1102	18-19	0.16190312202082424
1102	20-21	0.1866378257151453
1102	22-23	0.17064944531359316
1102	24-25	0.17209445695056758
1102	26-27	0.20272628253287195
1102	28-29	0.16955370170987294
1102	30-31	0.20489187641693718
1102	32-33	0.2091276919761853
1102	34-35	0.14693832120502037
1102	36-37	0.13800672795417057
1102	38-39	0.11922248002338875
1102	40-41	0.13978398321371088
1102	42-43	0.14308532696523457
1102	44-45	0.18725266973518728
1102	46-47	0.14117598892435268
1102	48-49	0.13583104025934745
1102	50-51	0.091758453687099
1102	52-53	0.11264739170414373
1102	54-55	0.06890655991012551
1102	56-57	0.0670513197553575
1102	58-59	0.1851859399343141
1102	60-61	0.08516707361025055
1102	62-63	0.11495550162928225
1102	64-65	0.09940612508695068
1102	66-67	0.07612597906059193
1102	68-69	0.11564886765776805
1102	70-71	0.14513497697328148
1102	72-73	0.13609995327517055
1102	74-75	0.0930250298033215
1102	76-77	0.10640719599293647
1102	78-79	0.09294828791162502
1102	80-81	0.058327214064540556
1102	82-83	0.014732687726819904
1102	84-85	0.06629388725698249
1102	86-87	0.07225008224838092
1102	88-89	0.02114950195712595
1102	90-91	0.04457194611486415
1102	92-93	0.04532392918714834
1102	94-95	0.028799741658488642
1102	96-97	0.037282722075417496
1102	98-99	0.05962953535862425
1102	100	0.0416142244867288
1103	1	0.0956263709230285
1103	2	0.11463676867759176
1103	3	0.08801406054416816
1103	4	0.0379237071055627
1103	5	0.0027933139220195358
1103	6	0.05997101064549071
1103	7	0.0935728719332829
1103	8	0.09192121980990464
1103	9	0.09855411750040588
1103	10-11	0.08927392412244473
1103	12-13	0.0868268563690151
1103	14-15	0.10549008435268803
1103	16-17	0.14739400447318474
1103	18-19	0.12228268813896648
1103	20-21	0.1248618150311529
1103	22-23	0.15397346938663503
1103	24-25	0.1351854607997538
1103	26-27	0.15943785203713645
1103	28-29	0.13204811707907282
1103	30-31	0.1196692999616289
1103	32-33	0.11115550056891266
1103	34-35	0.17960679198041873
1103	36-37	0.1438617529744235
1103	38-39	0.1350898643359315
1103	40-41	0.1515637139003303
1103	42-43	0.17323095118722875
1103	44-45	0.19860154638693928
1103	46-47	0.27361992700426896
1103	48-49	0.18637449377652615
1103	50-51	0.16600147016874445
1103	52-53	0.18356739091942842
1103	54-55	0.08671769186065603
1103	56-57	0.13994171737366656
1103	58-59	0.11632276903368677
1103	60-61	0.10712304728669153
1103	62-63	0.035079919985591346
1103	64-65	0.042915849469920886
1103	66-67	0.1073997264653812
1103	68-69	0.11702138205751567
1103	70-71	0.08421567734058044
1103	72-73	0.10108825099826646
1103	74-75	0.10070230088343379
1103	76-77	0.07970183389602425
1103	78-79	0.06765125217998502
1103	80-81	0.10647829873217773
1103	82-83	0.09140369994879194
1103	84-85	0.10520770571566374
1103	86-87	0.05663504736733671
1103	88-89	0.04813173589088393
1103	90-91	0.054561911361531656
1103	92-93	0.04740972170132096
1103	94-95	0.05574329496797503
1103	96-97	0.1036119419371957
1103	98-99	0.06352850526953802
1103	100	0.08912421019525851
1104	1	0.05759531163355547
1104	2	0.09712282280329276
1104	3	0.11161159282821842
1104	4	0.0857400331186966
1104	5	-0.0025862037660857595
1104	6	0.020039107661574462
1104	7	0.04467860323117634
1104	8	0.09092942387729863
1104	9	0.09535793985119767
1104	10-11	0.09370211859932454
1104	12-13	0.11456485591898513
1104	14-15	0.10957267222843114
1104	16-17	0.13193391252696784
1104	18-19	0.1000524954947224
1104	20-21	0.1170799997336971
1104	22-23	0.11928358913919368
1104	24-25	0.10184060773892867
1104	26-27	0.08108504244416537
1104	28-29	0.10286406369183965
1104	30-31	0.09394197752634881
1104	32-33	0.0997342877325238
1104	34-35	0.146648151230238
1104	36-37	0.1366563082393597
1104	38-39	0.1373005999299366
1104	40-41	0.10071465265172463
1104	42-43	0.09246074244374114
1104	44-45	0.11261311978252309
1104	46-47	0.11175159033766136
1104	48-49	0.12836449448757037
1104	50-51	0.1550582019008928
1104	52-53	0.18810855142587712
1104	54-55	0.16684154873161816
1104	56-57	0.17968770294960024
1104	58-59	0.13147489002003
1104	60-61	0.14020516012883633
1104	62-63	0.14528640149612926
1104	64-65	0.15436152916723245
1104	66-67	0.14580211817122546
1104	68-69	0.13293385529808432
1104	70-71	0.18204536718329223
1104	72-73	0.18423582946466155
1104	74-75	0.21291740558084626
1104	76-77	0.1385241837986797
1104	78-79	0.14302601421249506
1104	80-81	0.18861320212929655
1104	82-83	0.19790375790206127
1104	84-85	0.19629709897829883
1104	86-87	0.1141785390670762
1104	88-89	0.11126913692333318
1104	90-91	0.152797998157979
1104	92-93	0.11606503673749557
1104	94-95	0.033514624299016305
1104	96-97	0.11732561896269544
1104	98-99	0.2018052941180244
1104	100	0.24306293742081664
1105	1	0.050438082200258805
1105	2	0.07844259886273619
1105	3	0.061652969527905555
1105	4	0.02908197412590141
1105	5	0.04583480308419752
1105	6	0.06329168422478659
1105	7	0.0066140931641527345
1105	8	-0.01052901195067335
1105	9	0.062103738377615514
1105	10-11	0.013935828310209786
1105	12-13	0.023641119691895085
1105	14-15	0.05928784310733448
1105	16-17	0.07124457918583005
1105	18-19	0.06545362161162416
1105	20-21	0.10827435555575704
1105	22-23	0.1570262116635135
1105	24-25	0.03693588948983262
1105	26-27	0.06327730760073536
1105	28-29	0.06492797668306594
1105	30-31	0.10045695100789942
1105	32-33	0.029937987239328834
1105	34-35	0.06693581831806483
1105	36-37	0.04786447917542347
1105	38-39	0.03773116460654791
1105	40-41	0.11653253339500935
1105	42-43	0.09911864108354251
1105	44-45	0.1725477390831287
1105	46-47	0.1586907948337526
1105	48-49	0.07970350671760684
1105	50-51	0.10728348795938558
1105	52-53	0.13114411974616047
1105	54-55	0.09570427520068847
1105	56-57	0.06415902305648302
1105	58-59	0.03729559410134442
1105	60-61	0.04065012844309024
1105	62-63	0.008443362099029628
1105	64-65	-0.048193352630661934
1105	66-67	-0.07762776998453091
1105	68-69	-0.09889775627542008
1105	70-71	-0.013230937347415761
1105	72-73	-0.022968965731358537
1105	74-75	-0.04039534667973754
1105	76-77	-0.04078017451701754
1105	78-79	-0.048681170186924305
1105	80-81	-0.01579717483640053
1105	82-83	-0.04484271647004334
1105	84-85	-5.422674912054504E-4
1105	86-87	-0.05667058856760576
1105	88-89	-0.035925011958966024
1105	90-91	-0.05062540611027089
1105	92-93	-0.053228624626676435
1105	94-95	-0.024039948608375994
1105	96-97	-0.08902647807190789
1105	98-99	-0.09108795107105294
1105	100	-0.08367640872604909
1106	1	0.07758092234374914
1106	2	0.07726183806983755
1106	3	0.05634847062915327
1106	4	0.0545613100006932
1106	5	-0.0018199560903653378
1106	6	0.00805322787886098
1106	7	0.0326673664083188
1106	8	0.056259318692639226
1106	9	0.04620273621838322
1106	10-11	0.027534887812336706
1106	12-13	0.050256620057538726
1106	14-15	0.0849627374176194
1106	16-17	0.09175000754832041
1106	18-19	0.10553033590537098
1106	20-21	0.09769437645571344
1106	22-23	0.12870975046256916
1106	24-25	0.16789240309211806
1106	26-27	0.08549529886862217
1106	28-29	0.07067384104540508
1106	30-31	0.1020600920587782
1106	32-33	0.13407457146008284
1106	34-35	0.17786861934445142
1106	36-37	0.17718539838391223
1106	38-39	0.10536558094398885
1106	40-41	0.12886666976828565
1106	42-43	0.1450098585598809
1106	44-45	0.118101604059909
1106	46-47	0.18739447850452962
1106	48-49	0.16139644075943238
1106	50-51	0.14948827612559512
1106	52-53	0.14820638364808758
1106	54-55	0.14940787773810627
1106	56-57	0.16582739532662316
1106	58-59	0.1587728648617457
1106	60-61	0.1741938225422075
1106	62-63	0.1689480494435216
1106	64-65	0.14613718358510397
1106	66-67	0.21305798537784426
1106	68-69	0.19469725196772458
1106	70-71	0.17545626002515746
1106	72-73	0.23701416934810737
1106	74-75	0.22200837506962756
1106	76-77	0.1528767114332794
1106	78-79	0.17453865577661531
1106	80-81	0.199324406203921
1106	82-83	0.21645331859687644
1106	84-85	0.1791583558293368
1106	86-87	0.1857387638367527
1106	88-89	0.18422570136550576
1106	90-91	0.18257390393166162
1106	92-93	0.1898805570730957
1106	94-95	0.17544123061630046
1106	96-97	0.1789007755963965
1106	98-99	0.21094773895192276
1106	100	0.20443425500971557
1107	1	-0.01065779192099825
1107	2	0.0010097963155004663
1107	3	0.020830273640072505
1107	4	0.032387467567389194
1107	5	-0.06287608832837321
1107	6	-0.07921767661440526
1107	7	-0.078136520030057
1107	8	-0.03830641356452702
1107	9	0.010227571446598915
1107	10-11	0.008751554773965609
1107	12-13	0.01343396457449586
1107	14-15	0.05550991716800269
1107	16-17	0.048804379375077644
1107	18-19	0.015848209336901675
1107	20-21	0.04088176187542558
1107	22-23	0.056460562219321275
1107	24-25	0.040113951525285074
1107	26-27	0.07138509774866719
1107	28-29	0.0840963262130856
1107	30-31	0.0599568274380573
1107	32-33	0.08201871093545776
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2216	3	-0.14929237376735216
2216	4	-0.07894216274835486
2216	5	-0.04392750048666727
2216	6	-0.0951243862413591
2216	7	-0.1019536253516975
2216	8	-0.09907932000228925
2216	9	-0.13800365697466077
2216	10-11	-0.1714938719867405
2216	12-13	-0.11945460087411419
2216	14-15	-0.21484473797416825
2216	16-17	-0.17159440968627138
2216	18-19	-0.15776986375725244
2216	20-21	-0.17998011991107177
2216	22-23	-0.20517683292207067
2216	24-25	-0.241988532186852
2216	26-27	-0.2516301712372311
2216	28-29	-0.3221563121562667
2216	30-31	-0.2806069895164711
2216	32-33	-0.2460901944635694
2216	34-35	-0.23904219271472016
2216	36-37	-0.23509750577601807
2216	38-39	-0.24145481032660854
2216	40-41	-0.2726062034348615
2216	42-43	-0.2486458051104634
2216	44-45	-0.19358553947373025
2216	46-47	-0.24098900057347805
2216	48-49	-0.21170612002740086
2216	50-51	-0.15685062545433226
2216	52-53	-0.13661409144092573
2216	54-55	-0.129658530869456
2216	56-57	-0.12791683795569497
2216	58-59	-0.14820081548576525
2216	60-61	-0.14250088818008066
2216	62-63	-0.19458900445597038
2216	64-65	-0.18176813836834782
2216	66-67	-0.14689413951965236
2216	68-69	-0.18382330606564778
2216	70-71	-0.16611806258651285
2216	72-73	-0.13829347670603198
2216	74-75	-0.09091541494778
2216	76-77	-0.1346698677335354
2216	78-79	-0.14712426782290322
2216	80-81	-0.11670455328018647
2216	82-83	-0.12817913208080256
2216	84-85	-0.12489730093831497
2216	86-87	-0.14154957395232515
2216	88-89	-0.09169662048507377
2216	90-91	-0.13490731032365488
2216	92-93	-0.11394728843299617
2216	94-95	-0.155252498937557
2216	96-97	-0.13101138988354677
2216	98-99	-0.1415650876177068
2216	100	-0.12335918953913705
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25.0
3	13.0
4	20.0
5	42.0
6	109.0
7	208.0
8	460.0
9	888.0
10	1472.0
11	2513.0
12	3701.0
13	5792.0
14	6907.0
15	9179.0
16	11911.0
17	17382.0
18	20938.0
19	11569.0
20	7660.0
21	7225.0
22	7458.0
23	8016.0
24	9106.0
25	10838.0
26	13730.0
27	17628.0
28	22791.0
29	28953.0
30	37483.0
31	48387.0
32	62234.0
33	81266.0
34	115568.0
35	182439.0
36	356558.0
37	823866.0
38	1437615.0
39	694919.0
40	2443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35763773333737	19.19708160261949	13.387319060616862	24.05796160342628
2	15.592180797313715	19.923578317848143	40.214422067479525	24.26981881735862
3	18.493081506918493	25.1696663145186	29.869135094368556	26.468117084194354
4	12.44433009223456	16.514638024878487	38.06660557506342	32.974426307823535
5	12.907938738418927	37.79116004795787	35.39946166627784	13.901439547345367
6	31.45520708209561	37.65629208336364	17.36778574419606	13.520715090344687
7	27.726608192523795	32.50866458809938	22.029888804759914	17.734838414616913
8	25.59742520573583	36.1676319441987	19.809126689235683	18.425816160829786
9	25.601153022444866	16.18283711837771	20.633228524281172	37.582781334896254
10-11	24.4391656144776	26.30120851430248	28.451704398846584	20.80792147237334
12-13	25.26810428888225	23.604506117987512	28.915772494220153	22.211617098910086
14-15	22.211655318918357	25.411719113525276	26.225911300184958	26.150714267371406
16-17	21.65377095685954	29.039638641618044	26.71621148734725	22.590378914175172
18-19	21.587346249945476	27.563105024626427	29.336564022256855	21.512984703171238
20-21	22.501100923202742	26.790204339210167	29.105141114763377	21.60355362282371
22-23	22.128730906586572	26.54306235528079	29.240355073135998	22.087851664996645
24-25	22.07154267478812	26.628204739113343	29.21186428494985	22.088388301148683
26-27	21.90831199543011	26.801934166911284	29.148130777560606	22.141623060097995
28-29	21.769033913080023	26.847725991966993	29.223873121418464	22.15936697353452
30-31	21.973030341879447	27.0786115371884	28.95693943165994	21.99141868927221
32-33	21.902785030792867	26.79257559457961	29.278262557084076	22.026376817543447
34-35	21.990495580561724	26.724896035810264	29.2107816718216	22.07382671180641
36-37	22.208178366472612	26.976272233834624	28.837828739603687	21.97772066008908
38-39	22.040143220987556	27.0983886594227	28.835864289351328	22.02560383023842
40-41	21.930792748204098	26.998930436063013	28.89098437001062	22.17929244572227
42-43	22.018698494155736	26.852243993816654	28.85725880449293	22.27179870753468
44-45	22.134520402891162	27.073951048005675	28.633473060868734	22.158055488234425
46-47	22.205017508868107	27.25731676519437	28.356243252526482	22.181422473411036
48-49	22.24477148868291	27.290437132583666	28.036234152074464	22.42855722665896
50-51	22.29234471150219	27.324132690389334	27.93176490191303	22.451757696195447
52-53	22.245357422667947	27.389203347393853	27.943026868999254	22.422412360938946
54-55	22.298472426435804	27.566904301235667	27.907898316422685	22.22672495590584
56-57	22.174003649855674	27.353606431924486	27.91178712681258	22.56060279140726
58-59	22.022666517909144	27.097333619707946	28.216504614350217	22.66349524803269
60-61	22.328369843268362	27.388281540188736	27.935085821682726	22.348262794860172
62-63	22.102052687337302	27.31814773775321	28.01256299992308	22.567236574986403
64-65	22.14281229353183	27.1637126935018	28.06772764196624	22.62574737100013
66-67	22.004751211394257	27.23456102040706	28.01096410714628	22.749723661052403
68-69	22.30703834271608	27.30086953145418	27.91816842396794	22.4739237018618
70-71	22.276951991460912	27.150533637776547	27.915090274425257	22.65742409633728
72-73	22.1583165367419	27.331248934086094	27.85372491139706	22.656709617774947
74-75	22.297003401093068	27.31964868477962	27.71240858333661	22.670939330790706
76-77	22.280569829134812	27.15166527821807	27.777486981758486	22.79027791088863
78-79	22.318820635439423	27.331365759797116	27.82329190943919	22.52652169532427
80-81	22.274135493515203	27.26488694456793	27.811727214128666	22.6492503477882
82-83	22.20401401817369	27.263497382466568	27.837049666415925	22.695438932943816
84-85	22.151736357280562	27.292282815573703	27.66178170911669	22.894199118029043
86-87	22.262702825042577	27.263118744089127	27.694298394945665	22.779880035922627
88-89	22.092112868219495	27.289748242454742	27.66991078590189	22.94822810342387
90-91	22.099489127023777	27.176948223887642	27.7248053662182	22.998757282870383
92-93	22.17495103592501	27.26045693250207	27.648741432822767	22.915850598750158
94-95	21.976036735448155	27.12301244043219	27.775764060362047	23.125186763757608
96-97	22.079567750027522	27.031891386062313	27.77604666341632	23.112494200493845
98-99	22.025381686142524	27.20317341113191	27.671471246245066	23.099973656480504
100	22.081373952157023	27.02073470896309	27.614864625764753	23.28302671311514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	38.0
2	124.0
3	249.0
4	187.5
5	144.0
6	368.5
7	851.0
8	1567.5
9	1905.5
10	1596.0
11	1340.0
12	1409.0
13	1632.0
14	1896.5
15	2201.5
16	2553.5
17	2970.0
18	3445.5
19	3946.0
20	4664.5
21	5583.0
22	7111.5
23	9762.5
24	13255.0
25	17838.0
26	23788.5
27	31423.0
28	40864.0
29	50839.0
30	60470.0
31	70627.5
32	80350.5
33	89967.5
34	100751.0
35	109510.5
36	115438.0
37	122739.5
38	128806.0
39	131417.0
40	135645.5
41	139569.5
42	139479.0
43	138793.0
44	139216.0
45	138761.5
46	137458.5
47	134881.5
48	133353.5
49	132153.0
50	127707.0
51	124714.5
52	120794.0
53	115901.0
54	111727.5
55	105520.0
56	97637.5
57	88932.0
58	81684.0
59	76276.5
60	69700.5
61	60651.0
62	53324.0
63	46684.0
64	39977.0
65	32933.0
66	25986.0
67	19735.5
68	14371.5
69	11026.5
70	8130.0
71	5877.0
72	4531.0
73	3550.0
74	2705.0
75	1951.0
76	1383.5
77	1076.0
78	770.0
79	492.0
80	309.0
81	142.0
82	62.5
83	33.5
84	19.5
85	9.5
86	7.0
87	5.5
88	4.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09320985955365427
2	0.0011795605743673623
3	2.4574178632653383E-5
4	3.19464322224494E-4
5	0.038704331346429074
6	1.9659342906122706E-4
7	0.004005591117122501
8	3.9318685812245413E-4
9	1.720192504285737E-4
10-11	4.423352153877609E-4
12-13	0.0
14-15	6.143544658163346E-5
16-17	0.0
18-19	1.1058380384694022E-4
20-21	0.0
22-23	2.3345469701020714E-4
24-25	1.59732161112247E-4
26-27	0.0016341828790714498
28-29	0.008625536700061338
30-31	0.03731589025368416
32-33	0.02655240001258198
34-35	0.029599598163031
36-37	0.02711760612113301
38-39	0.025888897189500337
40-41	0.031921858043816746
42-43	0.023664934023245206
44-45	0.022559095984775804
46-47	0.016206670808234905
48-49	0.010898648223581775
50-51	0.007888311341081736
52-53	0.005799506157306198
54-55	0.0043987779752449555
56-57	0.002703159649591872
58-59	0.0012287089316326692
60-61	7.740866269285815E-4
62-63	0.0014990248965918564
64-65	0.00323150449019392
66-67	8.355220735102151E-4
68-69	0.001265570199581649
70-71	6.266415551326612E-4
72-73	8.355220735102151E-4
74-75	7.863737162449083E-4
76-77	5.406319299183745E-4
78-79	4.9148357265306766E-5
80-81	6.143544658163346E-4
82-83	5.652061085510278E-4
84-85	4.1776103675510753E-4
86-87	2.580288756428605E-4
88-89	0.0
90-91	7.372253589796015E-5
92-93	4.9148357265306766E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4069312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.37280815156321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.0531315145273	33.599742063798566
2	16.3739149460841	16.168523226460472
3	6.273725150098917	9.292542846944132
4	3.0790344457530714	6.080823079288847
5	1.7579898507256613	4.339844781613666
6	1.0583042288167686	3.135087099321502
7	0.7206173081565118	2.4905230074415154
8	0.4803775839999799	1.8974072236113957
9	0.3636406559690723	1.6158564308942647
>10	1.7122339327041363	14.738383557642177
>50	0.09176499092633253	3.0696346397306895
>100	0.033921082622966316	2.7388067115819896
>500	0.001145152656298477	0.3886223427900689
>1k	9.957847947293498E-5	0.11565993706055348
>5k	9.957847947293498E-5	0.3285430518202545
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7453	0.18315135334916566	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5912	0.1452825440762468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.2287089316326692E-5	0.0
14-15	0.0	0.0	0.0	2.4574178632653383E-5	0.0
16-17	0.0	0.0	0.0	6.143544658163346E-5	0.0
18-19	0.0	0.0	0.0	7.372253589796015E-5	0.0
20-21	0.0	0.0	0.0	7.372253589796015E-5	0.0
22-23	0.0	0.0	0.0	9.829671453061353E-5	0.0
24-25	0.0	0.0	0.0	1.5973216111224696E-4	0.0
26-27	0.0	0.0	0.0	3.19464322224494E-4	0.0
28-29	0.0	0.0	0.0	0.0010075413239387886	0.0
30-31	0.0	0.0	0.0	0.004693668118836796	0.0
32-33	0.0	0.0	0.0	0.01303660176462262	0.0
34-35	0.0	0.0	0.0	0.023861527452306436	0.0
36-37	0.0	0.0	0.0	0.04344714782253118	0.0
38-39	0.0	0.0	0.0	0.0788585392321847	0.0
40-41	0.0	0.0	0.0	0.1196885370303383	0.0
42-43	0.0	0.0	0.0	0.1613540569020021	0.0
44-45	0.0	0.0	0.0	0.2094088632181558	0.0
46-47	0.0	0.0	0.0	0.2689889593130239	0.0
48-49	0.0	0.0	0.0	0.33602731862290236	0.0
50-51	0.0	0.0	0.0	0.40799280074862776	0.0
52-53	0.0	0.0	0.0	0.482096236415394	0.0
54-55	0.0	0.0	0.0	0.5612005174339052	0.0
56-57	0.0	0.0	0.0	0.6577647523709167	0.0
58-59	0.0	0.0	0.0	0.7703144905084693	0.0
60-61	0.0	0.0	0.0	0.8880862416054606	0.0
62-63	0.0	0.0	0.0	1.008487429816146	0.0
64-65	0.0	0.0	0.0	1.1325993190003616	0.0
66-67	0.0	0.0	0.0	1.2731390466005066	0.0
68-69	0.0	0.0	0.0	1.4366802054008145	0.0
70-71	0.0	0.0	0.0	1.6159119772580721	0.0
72-73	0.0	0.0	0.0	1.8000954461098093	0.0
74-75	0.0	0.0	0.0	1.98727696475473	0.0
76-77	0.0	0.0	0.0	2.1945724486104776	0.0
78-79	0.0	0.0	0.0	2.424918512023654	0.0
80-81	0.0	0.0	0.0	2.6805882665177796	0.0
82-83	0.0	0.0	0.0	2.9473041143072836	0.0
84-85	0.0	0.0	0.0	3.21270524353994	0.0
86-87	0.0	0.0	0.0	3.493551244043219	0.0
88	0.0	0.0	0.0	3.7277063051444568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5720	0.0	47.964607	1
GTATCAA	10330	0.0	44.64513	1
TCAACGC	12500	0.0	36.442497	4
ATCAACG	12690	0.0	35.93391	3
CAACGCA	12895	0.0	35.362648	5
TATCAAC	13515	0.0	33.983875	2
AACGCAG	13430	0.0	33.944756	6
ACGCAGA	15415	0.0	29.268423	7
CGCAGAG	15915	0.0	28.259266	8
GTACATG	12345	0.0	25.578777	1
GCAGAGT	17735	0.0	25.014765	9
TACATGG	12595	0.0	23.96245	2
TATACCG	220	2.4501787E-9	23.505224	5
ACATGGG	13065	0.0	22.056952	3
AGAGTAC	16185	0.0	20.746513	10-11
GAGTACT	11760	0.0	20.660404	12-13
CAGAGTA	17325	0.0	19.991707	10-11
GTGGTAT	3135	0.0	19.964684	1
CATGGGG	8660	0.0	19.54245	4
GAGTACA	9110	0.0	19.37141	1
>>END_MODULE