FastQCFastQC Report
Wed 25 May 2016
SRR1033196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944878
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC31300.032.7227754
GGTATCA21600.032.71081
ATCAACG32400.031.4748333
CAACGCA33750.030.4865555
GTATCAA35850.029.694291
AACGCAG37250.027.7378066
GTACATG76550.025.8438851
TATCAAC40850.025.6648462
TACATGG74850.025.6267912
ACGCAGA43400.023.4825737
ACATGGG80050.023.0717183
CGCAGAG43600.023.0516998
GAGTACA56800.018.8248351
CATGGGG50000.018.6051524
GCAGAGT53100.017.8662159
AGTACAT58500.016.6356722
TGGACCG3402.0059815E-816.582135
GTATAAG11450.016.455391
TCTATAC12100.016.3122023
GGACCGT3252.1780579E-715.8958976
Produced by FastQC (version 0.11.5)