##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033196_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2944878
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718996508514106	33.0	31.0	34.0	30.0	34.0
2	31.827279092716235	34.0	31.0	34.0	30.0	34.0
3	31.9827109985541	34.0	31.0	34.0	30.0	34.0
4	35.31031472271517	37.0	35.0	37.0	33.0	37.0
5	35.421372634112515	37.0	35.0	37.0	35.0	37.0
6	35.4874008362995	37.0	37.0	37.0	35.0	37.0
7	35.42882387657485	37.0	37.0	37.0	35.0	37.0
8	35.41866046742853	37.0	37.0	37.0	35.0	37.0
9	37.082384397587944	39.0	38.0	39.0	35.0	39.0
10-11	37.07869714806522	39.0	38.0	39.0	35.0	39.0
12-13	37.02781473460021	39.0	38.0	39.0	35.0	39.0
14-15	38.49757765856514	41.0	39.0	41.0	35.0	41.0
16-17	38.51592357985628	41.0	39.0	41.0	35.0	41.0
18-19	38.523759558120915	41.0	39.0	41.0	34.0	41.0
20-21	38.55736400625085	41.0	39.0	41.0	34.0	41.0
22-23	38.528690662227774	41.0	39.0	41.0	34.0	41.0
24-25	38.52765666353581	41.0	39.0	41.0	34.0	41.0
26-27	38.486150529835186	41.0	39.0	41.0	34.0	41.0
28-29	38.45540239697536	40.5	39.0	41.0	34.0	41.0
30-31	38.45367227436926	41.0	39.0	41.0	34.0	41.0
32-33	38.51506412150181	41.0	39.0	41.0	34.0	41.0
34-35	38.483972510915564	41.0	39.0	41.0	34.0	41.0
36-37	38.412783313943734	41.0	39.0	41.0	34.0	41.0
38-39	38.33722975960295	41.0	39.0	41.0	34.0	41.0
40-41	38.283028023571774	41.0	39.0	41.0	33.5	41.0
42-43	38.18077268396178	41.0	38.0	41.0	33.0	41.0
44-45	38.0829905347522	40.0	38.0	41.0	33.0	41.0
46-47	37.97014103810073	40.0	38.0	41.0	33.0	41.0
48-49	37.864401513407344	40.0	38.0	41.0	33.0	41.0
50-51	37.75556627473192	40.0	38.0	41.0	33.0	41.0
52-53	37.61691418116472	40.0	37.5	41.0	32.0	41.0
54-55	37.47618797790605	40.0	37.0	41.0	32.0	41.0
56-57	37.3318412511486	40.0	37.0	41.0	32.0	41.0
58-59	37.14919327727668	40.0	36.5	41.0	31.0	41.0
60-61	36.93809658668373	40.0	36.0	41.0	31.0	41.0
62-63	36.68231994670068	39.0	35.5	41.0	31.0	41.0
64-65	36.42144852859779	39.0	35.0	41.0	31.0	41.0
66-67	36.13794917820025	39.0	35.0	41.0	30.5	41.0
68-69	35.832954540052256	38.0	35.0	40.0	30.0	41.0
70-71	35.48547664792905	37.0	35.0	40.0	30.0	41.0
72-73	35.118832596800274	37.0	35.0	39.5	30.0	41.0
74-75	34.747942868940584	36.0	34.5	39.0	29.0	41.0
76-77	34.40810739867662	36.0	34.0	39.0	29.0	40.5
78-79	34.0325590737545	35.0	34.0	37.5	29.0	39.5
80-81	33.66243253540554	35.0	34.0	37.0	29.0	39.0
82-83	33.4249204890661	35.0	34.0	37.0	29.0	39.0
84-85	33.141201265383486	35.0	34.0	36.0	29.0	38.0
86-87	32.87326520827009	35.0	34.0	36.0	28.5	37.0
88-89	32.50043346447629	35.0	34.0	36.0	27.5	37.0
90-91	32.30695974502169	35.0	34.0	35.0	27.0	36.5
92-93	32.1311010167484	35.0	34.0	35.0	27.0	36.0
94-95	31.963891373428712	35.0	34.0	35.0	27.0	36.0
96-97	31.79626507447847	35.0	34.0	35.0	26.5	36.0
98-99	31.612435727388366	35.0	33.0	35.0	25.5	36.0
100	31.45797313165435	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28048750963154845
1101	2	0.27265480601272785
1101	3	0.36197858458853815
1101	4	0.27459825683268946
1101	5	0.329249916628072
1101	6	0.27476237477741705
1101	7	0.3173228090023201
1101	8	0.28626710317700343
1101	9	0.3623058612190846
1101	10-11	0.3517783327276973
1101	12-13	0.3698251413488478
1101	14-15	0.4061503951724461
1101	16-17	0.3523239185853697
1101	18-19	0.3115350068133296
1101	20-21	0.31418112580966095
1101	22-23	0.2887549106840481
1101	24-25	0.30246724958502824
1101	26-27	0.32555765976296414
1101	28-29	0.34496865551300004
1101	30-31	0.339375117560337
1101	32-33	0.3511277508890487
1101	34-35	0.31557370054571265
1101	36-37	0.3555580127647744
1101	38-39	0.29907790138830137
1101	40-41	0.3402340184420254
1101	42-43	0.3175205790194724
1101	44-45	0.2820835000231128
1101	46-47	0.2530480598929614
1101	48-49	0.2073463736698713
1101	50-51	0.25098555086439234
1101	52-53	0.27623986452121585
1101	54-55	0.22745596788129063
1101	56-57	0.25169881650032266
1101	58-59	0.2421530725899501
1101	60-61	0.19982029175242388
1101	62-63	0.1656153241692948
1101	64-65	0.1934557715128804
1101	66-67	0.16236407875589265
1101	68-69	0.18647212579281813
1101	70-71	0.15503913466052666
1101	72-73	0.23384609301735537
1101	74-75	0.17176948323384522
1101	76-77	0.14190855546480918
1101	78-79	0.1266543766955195
1101	80-81	0.1586196448571684
1101	82-83	0.1891350083052714
1101	84-85	0.18188945430358672
1101	86-87	0.18262867149988438
1101	88-89	0.30773569583729454
1101	90-91	0.3783767163704752
1101	92-93	0.37700575860436203
1101	94-95	0.34372701502794456
1101	96-97	0.31009294417344435
1101	98-99	0.25579450311408536
1101	100	0.2911220517177462
1102	1	0.19192338373488838
1102	2	0.178418054022206
1102	3	0.29033303081823547
1102	4	0.17861115451532328
1102	5	0.08595837631805381
1102	6	0.15333073892922044
1102	7	0.1299841995366151
1102	8	0.11624659856209263
1102	9	0.16074460227554255
1102	10-11	0.17524522543464371
1102	12-13	0.13400799046610246
1102	14-15	0.157321079718443
1102	16-17	0.23164019484113396
1102	18-19	0.1930461741395888
1102	20-21	0.22613366889257946
1102	22-23	0.2403832921639406
1102	24-25	0.2629224826183716
1102	26-27	0.22925385678730947
1102	28-29	0.28831931212518214
1102	30-31	0.17130657611733113
1102	32-33	0.1795513536713571
1102	34-35	0.1946012618987112
1102	36-37	0.21110437116882252
1102	38-39	0.21597904733286555
1102	40-41	0.2555138947507629
1102	42-43	0.18757478194619637
1102	44-45	0.17083689041084682
1102	46-47	0.17614790643292366
1102	48-49	0.1761316389676253
1102	50-51	0.1331484689304645
1102	52-53	0.06710669877544007
1102	54-55	0.18464465736606428
1102	56-57	0.05749028107568677
1102	58-59	0.03898479654998965
1102	60-61	0.015840723085055686
1102	62-63	0.04316670904584896
1102	64-65	0.10114726693028331
1102	66-67	0.0483267411417998
1102	68-69	-0.005871635071507342
1102	70-71	0.15992634196775413
1102	72-73	0.11508395203204458
1102	74-75	0.13551797611435745
1102	76-77	0.13774666969804628
1102	78-79	0.16492890772494917
1102	80-81	0.15561899483750352
1102	82-83	0.1145318506465074
1102	84-85	0.1383773458481059
1102	86-87	0.1532877418130809
1102	88-89	0.25087016970076803
1102	90-91	0.25056905987167966
1102	92-93	0.25850855905525094
1102	94-95	0.2699828758077558
1102	96-97	0.22452724674678493
1102	98-99	0.24201587505660882
1102	100	0.15044614090401964
1103	1	0.19647959872740728
1103	2	0.1951574009014969
1103	3	0.20871463530809464
1103	4	0.21069049460866296
1103	5	0.16898958616548043
1103	6	0.1702767238109928
1103	7	0.21514113873553953
1103	8	0.15014540990688374
1103	9	0.23276592798487172
1103	10-11	0.237533094699252
1103	12-13	0.2471798977098274
1103	14-15	0.24879844409661445
1103	16-17	0.24427359176965524
1103	18-19	0.25791885398139414
1103	20-21	0.2549201781843351
1103	22-23	0.23463689233749108
1103	24-25	0.2049628312609002
1103	26-27	0.29769809233793865
1103	28-29	0.31380011398075425
1103	30-31	0.2889445062887006
1103	32-33	0.2581436288030403
1103	34-35	0.27574945166081477
1103	36-37	0.2846994552424036
1103	38-39	0.26348276014219607
1103	40-41	0.29009319312713444
1103	42-43	0.195669651872727
1103	44-45	0.1837571331479282
1103	46-47	0.18331919070340064
1103	48-49	0.21604233686453256
1103	50-51	0.17910951641548678
1103	52-53	0.10769468540376437
1103	54-55	0.1363927648067076
1103	56-57	0.13767225044988152
1103	58-59	0.10343073971481687
1103	60-61	0.07917507616626551
1103	62-63	0.14783795159937085
1103	64-65	0.2173447383263536
1103	66-67	0.22207036539993652
1103	68-69	0.20954812096772457
1103	70-71	0.1695461057941401
1103	72-73	0.20377245428198165
1103	74-75	0.16463466122918646
1103	76-77	0.12098070739905609
1103	78-79	0.15176420360942444
1103	80-81	0.17617399725708793
1103	82-83	0.11351518054977561
1103	84-85	0.09677485840491329
1103	86-87	0.13433386408271275
1103	88-89	0.23360463291474787
1103	90-91	0.25546721486965396
1103	92-93	0.22913623241701941
1103	94-95	0.25301475312652144
1103	96-97	0.30599877075071547
1103	98-99	0.3576788131542017
1103	100	0.3729196784465003
1104	1	0.0645095243152305
1104	2	0.05846480515408459
1104	3	0.12509120699075638
1104	4	0.06564021683534804
1104	5	0.07191303644754043
1104	6	0.04098168408700076
1104	7	0.02512506699127215
1104	8	-0.0016320178640185645
1104	9	0.03310230959237259
1104	10-11	0.03386230830880521
1104	12-13	0.00806456687297441
1104	14-15	0.045150292079341625
1104	16-17	0.03669378216624608
1104	18-19	-0.009184455190748508
1104	20-21	-0.05449210491408252
1104	22-23	0.003763098387963737
1104	24-25	-0.00561580510516535
1104	26-27	-0.001240154663335602
1104	28-29	0.04192290746419758
1104	30-31	0.0707341485852453
1104	32-33	0.010149355701244644
1104	34-35	0.00880868139569202
1104	36-37	0.1250408994493455
1104	38-39	-0.06243324892226099
1104	40-41	-0.028255756584378844
1104	42-43	-0.036746627732767934
1104	44-45	-0.047422800743632365
1104	46-47	-0.03354636379855691
1104	48-49	-0.10009511404967952
1104	50-51	-0.09971871949628763
1104	52-53	-0.174639414016049
1104	54-55	-0.13323179924723405
1104	56-57	-0.19771909652300224
1104	58-59	-0.1929075008577712
1104	60-61	-0.12726026452792638
1104	62-63	-0.23677340683802583
1104	64-65	-0.2644814939746354
1104	66-67	-0.22975538474873503
1104	68-69	-0.2885790386729141
1104	70-71	-0.23980748865146495
1104	72-73	-0.19076628665757056
1104	74-75	-0.22156820168418534
1104	76-77	-0.2516167811230474
1104	78-79	-0.166013342020932
1104	80-81	-0.15554862586586893
1104	82-83	-0.15070922385816488
1104	84-85	-0.0809860261838864
1104	86-87	-0.07129507684291525
1104	88-89	-0.05835976281445454
1104	90-91	-0.047800733250760175
1104	92-93	-0.06714184625951702
1104	94-95	-0.08799348096378878
1104	96-97	-0.14108980598918208
1104	98-99	-0.06453142668979694
1104	100	-0.022019650718892336
1105	1	-0.04480356499905014
1105	2	-0.017804498695483062
1105	3	0.054975308844102955
1105	4	-0.07122526553850861
1105	5	-0.047529804313136026
1105	6	-0.059082810860054735
1105	7	-0.02639489342064394
1105	8	-0.036428637583043155
1105	9	-0.1348149144136528
1105	10-11	-0.07890707501395156
1105	12-13	-0.04960974723226741
1105	14-15	-0.06634096318318683
1105	16-17	-0.07807293793547387
1105	18-19	-0.10869129478349748
1105	20-21	-0.09559475679083818
1105	22-23	0.01855119567326824
1105	24-25	-0.04701960193312971
1105	26-27	-0.02589714803593779
1105	28-29	0.026865106909369274
1105	30-31	0.0058382015918141406
1105	32-33	-0.02378111678665107
1105	34-35	-0.0938845909376198
1105	36-37	-0.07887776107813949
1105	38-39	-0.07283831258139628
1105	40-41	-0.07719915808138467
1105	42-43	-0.14512468251334099
1105	44-45	-0.13534496061968326
1105	46-47	-0.08551488166928323
1105	48-49	-0.12445973138229505
1105	50-51	-0.013871789610604424
1105	52-53	-0.04174609042805599
1105	54-55	-0.06976210381443337
1105	56-57	-0.05935176035391976
1105	58-59	-0.060489199460654675
1105	60-61	-0.11294995440550792
1105	62-63	-0.11035762267142957
1105	64-65	-0.1121993966785908
1105	66-67	-0.08649238914330226
1105	68-69	-0.17275183330753663
1105	70-71	-0.20071994837371676
1105	72-73	-0.16570995232790864
1105	74-75	-0.18184448084713267
1105	76-77	-0.11836867212689839
1105	78-79	-0.07781831890734736
1105	80-81	-0.20358993549564275
1105	82-83	-0.1764959847930001
1105	84-85	-0.17854292851780684
1105	86-87	-0.057241672119580755
1105	88-89	-0.01186968148004297
1105	90-91	-0.0664571053736438
1105	92-93	-0.07185572853617828
1105	94-95	-0.08869088590326157
1105	96-97	-0.05328085196703469
1105	98-99	-0.031225600877974102
1105	100	0.06268882169253231
1106	1	0.19878828927471304
1106	2	0.1869014839550367
1106	3	0.14534099739020334
1106	4	0.20202610751986327
1106	5	0.17306501822101694
1106	6	0.19891598061368398
1106	7	0.12556958951519448
1106	8	0.17101961428614487
1106	9	0.19725106609845255
1106	10-11	0.17213615977021846
1106	12-13	0.17970477051350287
1106	14-15	0.2786602275229839
1106	16-17	0.2106967487103475
1106	18-19	0.24079534480472375
1106	20-21	0.21462829609069445
1106	22-23	0.24797592461548845
1106	24-25	0.2664200788766138
1106	26-27	0.2719320305833506
1106	28-29	0.28393451981428086
1106	30-31	0.21581022404674854
1106	32-33	0.2563542288401166
1106	34-35	0.2325447222233663
1106	36-37	0.1938095959308015
1106	38-39	0.19474287920208155
1106	40-41	0.1593450521503499
1106	42-43	0.14628956659454673
1106	44-45	0.17047237816821337
1106	46-47	0.2349011628129034
1106	48-49	0.22015412544055124
1106	50-51	0.2344603104913361
1106	52-53	0.15654126706689198
1106	54-55	0.20497228568337533
1106	56-57	0.18012451320556266
1106	58-59	0.12962308939656708
1106	60-61	0.1609394488360465
1106	62-63	0.20720330888200067
1106	64-65	0.21849372796526012
1106	66-67	0.17598152365894038
1106	68-69	0.16545600405009253
1106	70-71	0.22644756211482786
1106	72-73	0.22906670521406625
1106	74-75	0.25113506290179544
1106	76-77	0.2338273302593734
1106	78-79	0.2483610067074551
1106	80-81	0.23650038199764367
1106	82-83	0.2237541242538228
1106	84-85	0.2500830957831255
1106	86-87	0.275141020034674
1106	88-89	0.32771085987006643
1106	90-91	0.34799723732211874
1106	92-93	0.27659395380042895
1106	94-95	0.34870748388228634
1106	96-97	0.317376816767986
1106	98-99	0.31501768064343594
1106	100	0.37875134672672317
1107	1	0.05891469432896912
1107	2	0.07010825991153169
1107	3	0.1005227684610297
1107	4	0.08495286920340561
1107	5	0.12995249429097555
1107	6	0.07047328777086648
1107	7	0.10623781246489727
1107	8	0.0385243954326242
1107	9	0.13255634485363288
1107	10-11	0.11002435143220879
1107	12-13	0.06482381060902753
1107	14-15	0.04349823732154334
1107	16-17	0.033917734418920986
1107	18-19	0.08256963385528593
1107	20-21	0.06426143364090109
1107	22-23	0.08174727448845687
1107	24-25	0.08036068182124012
1107	26-27	0.13646178161371836
1107	28-29	0.05057378462124973
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2216	20-21	-0.3633521343694781
2216	22-23	-0.39147554607450274
2216	24-25	-0.414738841588985
2216	26-27	-0.462020935596982
2216	28-29	-0.40097057350600096
2216	30-31	-0.375956750039137
2216	32-33	-0.39281470997723744
2216	34-35	-0.39511940700568005
2216	36-37	-0.32165185224913273
2216	38-39	-0.3458544559218524
2216	40-41	-0.38150816282340827
2216	42-43	-0.36693929072927745
2216	44-45	-0.3866065122313387
2216	46-47	-0.29202858716953983
2216	48-49	-0.27410959901631315
2216	50-51	-0.28761643552219596
2216	52-53	-0.26250131436183466
2216	54-55	-0.2447770580812758
2216	56-57	-0.2586428142147099
2216	58-59	-0.2449719839065878
2216	60-61	-0.1631679955485552
2216	62-63	-0.13939677479883983
2216	64-65	-0.10392660053957314
2216	66-67	-0.10941108269033606
2216	68-69	-0.08946913043758542
2216	70-71	-0.21031762297968726
2216	72-73	-0.10779060778611438
2216	74-75	-0.08333215674977623
2216	76-77	-0.1748596393420243
2216	78-79	-0.19054654707688456
2216	80-81	-0.119994631060095
2216	82-83	-0.09889789752105571
2216	84-85	-0.13124219916438307
2216	86-87	-0.1512370055197465
2216	88-89	-0.1750469896730138
2216	90-91	-0.18482946692824953
2216	92-93	-0.12523362022253082
2216	94-95	-0.1564925878490584
2216	96-97	-0.15761011775751044
2216	98-99	-0.1583305540839035
2216	100	-0.17763969622949816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26751.0
3	4778.0
4	3013.0
5	2455.0
6	2167.0
7	2659.0
8	2982.0
9	3205.0
10	3582.0
11	3892.0
12	4343.0
13	4995.0
14	5580.0
15	5802.0
16	5956.0
17	5910.0
18	5855.0
19	6006.0
20	5864.0
21	6114.0
22	7205.0
23	9273.0
24	14492.0
25	20704.0
26	22163.0
27	19198.0
28	19331.0
29	24330.0
30	29655.0
31	35580.0
32	47050.0
33	64371.0
34	89950.0
35	135710.0
36	252442.0
37	556555.0
38	993680.0
39	489916.0
40	1364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72543828479288	20.009375695213592	13.461822148649057	23.803363871344477
2	15.197082510463437	20.184821479870525	41.10965641576742	23.50843959389862
3	18.091773856224698	25.42637687118055	30.04102963137892	26.440819641215835
4	11.86121642003995	16.78352560705502	39.824847471906295	31.530410500998734
5	12.502458706470703	38.24116623709371	36.31235135229705	12.944023704138544
6	30.538771673395317	38.77330348458751	17.99900428713479	12.688920554882388
7	26.941468561557052	32.61452559878099	22.893607512638848	17.550398327023107
8	24.364639551750404	37.16724895234427	20.769989176176992	17.698122319728327
9	24.508908223796155	17.269333416656536	21.412138062015558	36.80962029753175
10-11	23.32451561266403	27.279889126658457	29.45598733535976	19.93960792531775
12-13	24.03958687818612	24.601923645144527	29.35836041855584	22.00012905811351
14-15	21.32341803148959	26.7550974106913	26.35032051347366	25.57116404434545
16-17	21.061203903477825	30.132298111858603	26.839730020488755	21.966767964174817
18-19	20.874252668452936	29.21536088773291	28.802445667217675	21.10794077659648
20-21	22.207635922331743	28.500527993864473	28.205333740013717	21.08650234379007
22-23	21.652539408360067	28.283984722319396	28.342281313913137	21.721194555407404
24-25	21.629264384131385	28.362320590556457	28.24641232061603	21.76200270469613
26-27	21.36971115416574	28.267276518545952	28.398019713540112	21.964992613748194
28-29	21.36393617006824	28.32424352754656	28.483838735139205	21.827981567245995
30-31	21.650742952862963	28.903132613761816	27.99131949427003	21.45480493910519
32-33	21.266721419720884	28.481166579713925	28.548979528063995	21.703132472501196
34-35	21.34773590922784	28.21944643219207	28.692169987573134	21.740647671006958
36-37	21.781169826718312	28.59741319572091	28.058222538658494	21.56319443890228
38-39	21.528097702670877	28.753929592769122	28.199452230093247	21.518520474466754
40-41	21.268023633722137	28.477867667185354	28.5039832154115	21.75012548368101
42-43	21.30914608489943	28.319008421071935	28.52414406257885	21.847701431449785
44-45	21.422457377813284	28.526831537828702	28.438178408267955	21.61253267609006
46-47	21.474782329929365	28.694533122170824	28.32683256405724	21.50385198384257
48-49	21.406037440088454	28.54273593959164	28.331615025750846	21.71961159456906
50-51	21.456242249835338	28.428423244440726	28.310703093070234	21.804631412653702
52-53	21.445386295863752	28.481991039412524	28.326235205063515	21.74638745966021
54-55	21.645640610045135	28.79305663868003	28.137527915747036	21.423774835527794
56-57	21.45538977439141	28.561343039667214	28.215989982384276	21.767277203557104
58-59	21.234483090004233	28.16652271291388	28.658710413213207	21.94028378386868
60-61	21.579665228929006	28.581050897203653	28.199172877780526	21.64011099608682
62-63	21.47215305610478	28.551982688142925	28.207273662664985	21.76859059308731
64-65	21.394733486517907	28.302131866754888	28.379844767758637	21.923289878968568
66-67	21.264711590263545	28.349688835975055	28.29834460704771	22.087254966713694
68-69	21.762502589318764	28.482625246622	28.13117403957498	21.623698124484257
70-71	21.64404862182087	28.263356254453807	28.250350319774505	21.842244803950816
72-73	21.46778675719394	28.38857974923098	28.353874759471836	21.789758734103238
74-75	21.46679966890931	28.4584841989091	28.316132181590508	21.75858395059108
76-77	21.525064402225773	28.446002986986436	28.159308660019413	21.86962395076838
78-79	21.704578583201194	28.564787294426946	28.15499527724675	21.575638845125106
80-81	21.623034569036538	28.28434372213284	28.289912910124855	21.802708798705776
82-83	21.42329944605981	28.405645520725002	28.42474690663667	21.74630812657852
84-85	21.4990080800219	28.268479900461628	28.30712436931072	21.925387650205753
86-87	21.619050901663964	28.405254608496218	28.165968856007638	21.809725633832183
88-89	21.43826003096107	28.266041774383893	28.23451019899567	22.06118799565937
90-91	21.447219644156952	28.178318866842517	28.327143414283157	22.04731807471737
92-93	21.605973528952557	28.36722471487475	28.20485971014473	21.82194204602796
94-95	21.371268567493377	28.13606346595032	28.370979576611276	22.121688389945028
96-97	21.40123829057762	28.12432162762572	28.36054679353927	22.113893288257383
98-99	21.42485553772893	28.199680896958956	28.28739836745764	22.088065197854476
100	21.60129364895289	28.094610718307873	28.090477545428183	22.21361808731106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	320.0
1	288.5
2	270.5
3	262.0
4	218.0
5	182.5
6	149.5
7	155.5
8	179.0
9	179.0
10	160.5
11	160.0
12	184.5
13	205.5
14	243.5
15	311.5
16	440.5
17	647.5
18	923.5
19	1331.0
20	1988.5
21	3098.5
22	4875.0
23	7469.5
24	10695.0
25	14806.5
26	20471.0
27	27570.5
28	36179.5
29	45273.5
30	53956.0
31	63333.0
32	72067.5
33	80318.0
34	88966.0
35	95642.5
36	99990.5
37	104739.0
38	108139.5
39	108868.5
40	109365.0
41	109345.0
42	107872.0
43	105815.5
44	103373.5
45	100298.5
46	97781.0
47	94794.0
48	91991.5
49	89954.5
50	87121.5
51	83277.0
52	77519.5
53	72640.0
54	69542.5
55	65977.5
56	61052.0
57	55293.5
58	50191.0
59	45072.5
60	39692.5
61	33780.5
62	28422.0
63	23914.5
64	19634.5
65	15783.0
66	12376.0
67	9299.5
68	7038.0
69	5520.0
70	4102.5
71	3025.5
72	2284.5
73	1770.0
74	1389.0
75	997.0
76	686.0
77	535.5
78	404.0
79	293.0
80	216.0
81	111.5
82	55.0
83	42.0
84	25.5
85	17.5
86	14.5
87	16.0
88	12.0
89	4.5
90	6.0
91	7.5
92	5.0
93	4.0
94	3.0
95	2.5
96	1.0
97	1.5
98	2.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32697449605722206
2	0.11698277483821061
3	0.06394152830779407
4	0.03932251183240868
5	0.042684280978702686
6	0.008625145082410884
7	0.0063500083874442345
8	0.015178897054478996
9	0.01765777733406953
10-11	0.020968610584207563
12-13	0.015891999600662576
14-15	0.018336922616149123
16-17	0.019712191812360308
18-19	0.01932168327516454
20-21	0.0242794438343456
22-23	0.015348683374998895
24-25	0.025111396804893108
26-27	0.018897217473864792
28-29	0.018812324313604842
30-31	0.01967823454825633
32-33	0.025467948077984894
34-35	0.015314726110894917
36-37	0.009491055317062371
38-39	0.013362183424916074
40-41	0.00923637583628252
42-43	0.008183700649059147
44-45	0.008676080978566853
46-47	0.007691320319551438
48-49	0.004074871692477583
50-51	0.006197200698976325
52-53	0.0069612391413158715
54-55	0.002699602496266399
56-57	0.0022242007988106808
58-59	0.0022581580629146606
60-61	0.0030391751373061975
62-63	0.0025637734398504795
64-65	0.002190243534706701
66-67	0.0016469273090430232
68-69	0.0029882392411502276
70-71	0.0023260725911226202
72-73	0.0019185854218748619
74-75	0.0022242007988106808
76-77	0.0035315554668139053
78-79	0.003107089665514157
80-81	0.0035315554668139053
82-83	0.0022242007988106808
84-85	0.0028524101847343082
86-87	0.0024619016475385396
88-89	0.5994645618596085
90-91	0.6092442539215547
92-93	0.6054919762380648
94-95	0.5925542586144485
96-97	0.5899904851745981
98-99	0.5873587972065396
100	0.5889887458835307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2944878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.2646985726111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86608325234967	53.83769400824101
2	13.340688426510386	18.213961483737055
3	3.9374081917017545	8.063579500915498
4	1.5376391817074069	4.1986590101077
5	0.7397501487465951	2.5249410461615267
6	0.4227434897175683	1.7315074139462108
7	0.2597282547970445	1.2411189717157398
8	0.17411756802107628	0.9508866637723904
9	0.12493069203122309	0.7675520430581234
>10	0.5648848616596591	6.610371915706489
>50	0.025584255247760944	1.1754705880627085
>100	0.006441677509783596	0.6842573545755725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.3957264103979856E-5	0.0
4	0.0	0.0	0.0	3.3957264103979856E-5	0.0
5	0.0	0.0	0.0	6.791452820795971E-5	0.0
6	0.0	0.0	0.0	6.791452820795971E-5	0.0
7	0.0	0.0	0.0	1.0187179231193958E-4	0.0
8	0.0	0.0	0.0	1.0187179231193958E-4	0.0
9	0.0	0.0	0.0	2.0374358462387916E-4	0.0
10-11	0.0	0.0	0.0	2.3770084872785902E-4	0.0
12-13	0.0	0.0	0.0	2.7165811283183885E-4	0.0
14-15	3.3957264103979856E-5	0.0	0.0	3.0561537693581873E-4	0.0
16-17	6.791452820795971E-5	0.0	0.0	3.225940089878087E-4	0.0
18-19	6.791452820795971E-5	0.0	0.0	3.395726410397986E-4	0.0
20-21	8.489316025994965E-5	0.0	0.0	3.7352990514377845E-4	0.0
22-23	1.697863205198993E-4	0.0	0.0	3.9050853719576836E-4	0.0
24-25	2.3770084872785902E-4	0.0	0.0	5.433162256636777E-4	0.0
26-27	2.5467948077984894E-4	0.0	0.0	6.451880179756174E-4	0.0
28-29	2.7165811283183885E-4	0.0	0.0	0.0013582905641591945	0.0
30-31	2.8863674488382876E-4	0.0	0.0	0.0047200597104532	0.0
32-33	3.0561537693581873E-4	0.0	0.0	0.013243333000552145	0.0
34-35	3.0561537693581873E-4	0.0	0.0	0.024941610484373204	0.0
36-37	3.0561537693581873E-4	0.0	0.0	0.045570648427540966	0.0
38-39	3.0561537693581873E-4	0.0	0.0	0.0810899466803039	0.0
40-41	3.0561537693581873E-4	0.0	0.0	0.12036152261655661	0.0
42-43	3.225940089878087E-4	0.0	0.0	0.16058390194772076	0.0
44-45	3.7352990514377845E-4	0.0	0.0	0.20635829395988559	0.0
46-47	3.7352990514377845E-4	0.0	0.0	0.2633555617584158	0.0
48-49	3.7352990514377845E-4	0.0	0.0	0.3279422780841855	0.0
50-51	3.7352990514377845E-4	0.0	0.0	0.3976565412896561	0.0
52-53	3.7352990514377845E-4	0.0	0.0	0.46791412072079047	0.0
54-55	3.7352990514377845E-4	0.0	0.0	0.5436388196726656	0.0
56-57	3.7352990514377845E-4	0.0	0.0	0.6317748986545453	0.0
58-59	4.414444333517382E-4	0.0	0.0	0.7354634045960478	0.0
60-61	4.7540169745571804E-4	0.0	0.0	0.8449586026993309	0.0
62-63	4.7540169745571804E-4	0.0	0.0	0.957679740892492	0.0
64-65	5.093589615596979E-4	0.0	0.0	1.0778544985564769	0.0
66-67	5.093589615596979E-4	0.0	0.0	1.2176905121366657	0.0
68-69	5.093589615596979E-4	0.0	0.0	1.3744881791367929	0.0
70-71	5.433162256636777E-4	0.0	0.0	1.5430690167810008	0.0
72-73	5.433162256636777E-4	0.0	0.0	1.7222445208256505	0.0
74-75	5.433162256636777E-4	0.0	0.0	1.9027443581703554	0.0
76-77	5.772734897676576E-4	1.6978632051989928E-5	0.0	2.0954348533283893	0.0
78-79	5.772734897676576E-4	3.3957264103979856E-5	0.0	2.316208005900414	0.0
80-81	5.772734897676576E-4	3.3957264103979856E-5	0.0	2.566031597913394	0.0
82-83	5.772734897676576E-4	3.3957264103979856E-5	0.0	2.815193023276346	0.0
84-85	5.772734897676576E-4	3.3957264103979856E-5	0.0	3.0653392092983136	0.0
86-87	5.772734897676576E-4	3.3957264103979856E-5	0.0	3.3377104246763363	0.0
88	5.772734897676576E-4	3.3957264103979856E-5	0.0	3.5645279702588697	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3130	0.0	32.722775	4
GGTATCA	2160	0.0	32.7108	1
ATCAACG	3240	0.0	31.474833	3
CAACGCA	3375	0.0	30.486555	5
GTATCAA	3585	0.0	29.69429	1
AACGCAG	3725	0.0	27.737806	6
GTACATG	7655	0.0	25.843885	1
TATCAAC	4085	0.0	25.664846	2
TACATGG	7485	0.0	25.626791	2
ACGCAGA	4340	0.0	23.482573	7
ACATGGG	8005	0.0	23.071718	3
CGCAGAG	4360	0.0	23.051699	8
GAGTACA	5680	0.0	18.824835	1
CATGGGG	5000	0.0	18.605152	4
GCAGAGT	5310	0.0	17.866215	9
AGTACAT	5850	0.0	16.635672	2
TGGACCG	340	2.0059815E-8	16.58213	5
GTATAAG	1145	0.0	16.45539	1
TCTATAC	1210	0.0	16.312202	3
GGACCGT	325	2.1780579E-7	15.895897	6
>>END_MODULE